Using protein blotting and an immuno-overlay procedure, we have reexamined the cross-reacting material produced by ADH null-activity mutants generated with ethyl methanesulfonate (EMS). Of the 13 mutants, 11 have an immunodetectable polypeptide of wild-type size. The native and urea denatured isoelectric points (pI) establish that 7 of 13 of the mutations have no effect on protein charge. The e...

INTRODUCTION The aim of this study was to examine the potential effect of chorionic villus sampling (CVS) on placental perfusion by examining the change in uterine artery pulsatility index (PI) between the first and second trimesters of pregnancy. MATERIALS AND METHODS This was a prospective screening study for pregnancy complications which included measurement of uterine artery PI at 11(+0) ...

OBJECTIVE To investigate the maternal serum concentration of insulin-like growth factor-I (IGF-I) in the first trimester of pregnancies that subsequently develop preeclampsia (PE) and to examine the possible association with uterine artery pulsatility index (PI). METHODS The maternal serum concentration of IGF-I and uterine artery PI at 11-13 weeks were measured in 53 cases that developed PE,...

Root-knot nematodes (Meloidogynespp.) are the most important pests of vegetables, field crops, and ornamental plants. During our previous study, twenty eight okra cultivars were examined against resistance and Pusa Swami was ranked the most susceptible. The purpose of this study was to investigate the effects of increasing initial population density (Pi) of M. incognita on nematode reproduction...

The spatial and temporal dynamics of Meloidogyne incognita, relative to soybean shoot and root growth in field microplots, were determined at 11 sampling dates during a growing season. The population dynamics of M. incognita on soybean were dependent on initial population (Pi), soil moisture, and root spatial distribution. Final egg and juvenile population densities were greatest in plots with ...

The uptake of [32P]phosphate by human, gel-filtered blood platelets and its incorporation into cytoplasmic ATP and polyphosphoinositides was studied. In unstimulated platelets, uptake was Na+o-dependent and saturable at approximately 20 nmol/min/10(11) cells with a half-maximal rate at 0.5 mM extracellular phosphate. Upon stimulation with thrombin or collagen, net influx of [32P]Pi was accelera...

OBJECTIVES To assess the prevalence of hepatitis C virus (HCV) NS3/4A protease inhibitor (PI) resistance mutations in HCV genotype 1-infected PI-naive individuals in Italy. PATIENTS AND METHODS One hundred and twelve patients infected with HCV genotype 1a or 1b (based on Versant HCV Genotype 2.0 or 5'UTR/core sequencing) and never treated with any HCV PI were evaluated. The whole NS3 region w...

OBJECTIVE To evaluate the role of cervical length (CL) and uterine artery pulsatility index (UtA-PI) at 11+0 to 13+6 weeks as predictors of spontaneous preterm delivery (sPTD) in a Chilean population. METHODS This was a prospective study of asymptomatic women with singleton pregnancies attending for a nuchal translucency scan at 11+0 to 13+6 weeks' gestation and who underwent a transvaginal s...

We examined the role of phosphatidylinositol 3-kinase (PI 3-K) in FMLP-stimulated cell-cell adhesion of human neutrophils. The specific PI 3-K inhibitors wortmannin and LY294002 inhibited neutrophil homotypic aggregation stimulated by chemoattractants such as FMLP (50% inhibitory concentration (IC50) approximately 11 nM and 13 microM, respectively) but not PMA. Wortmannin also inhibited FMLP-st...