In k 2 experimental design, the classical fractional factorial designs, the Plackett-Burman designs and the Taguchi orthogonal-array designs all require, with few exceptions, n 2 test conditions, for some k n ≤ . Tsao and Wibowo (2005) pointed out that when only m of the k 2 effects/interactions are non-zero, only m test conditions are required for their estimation. Given the identities of m pr...

The Plackett-Burman design and the Box-Behnken design, statistical methodologies, were employed for the optimization lipase and biosurfactant production by Ochrobactrum intermedium strain MZV101 in an identical broth medium for detergent applications. Environmental factor pH determined to be most mutual significant variables on production. A high concentration of molasses at high temperature an...

The nutritional medium requirement for alkaline protease production was optimized by newly isolated Bacillus amyloliquefaciens B7. The variables significantly influencing the protease activity were screened through one-factor-at-a-time experiments and Plackett–Burman design, by which fructose and yeast extract were identified as the most significant variables. The steepest ascent method was emp...

Production of tannase by Aspergillus flavus (MTCC 3783) using tamarind seed powder as substrate was studied in submerged fermentation. Plackett-Burman design was applied for the screening of 12 medium nutrients. From the results, the significant nutrients were identified as tannic acid, magnesium sulfate, ferrous sulfate and ammonium sulfate. Further the optimization of process parameters was c...

Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett– –Burman design...

Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum producti...

The Plackett-Burman design and support vector machine (SVM) were reported to be used on many fields such as some feature selections, protein structure prediction, or forecasting of other situations. Here, with suspension adapted Chinese hamster ovary (CHO) cells as the object of study, a serum-free medium for the culture of CHO cells in suspension was optimized by this method. Support vector ma...

The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9. involves optimization process parameters for enhanced production under submerged fermentation condition. During process, maximum (92.12 µg/ml) obtained with carbon source mannitole, nitrogen peptone, inorganic salt NaCl and metal ion FeSO4. Plackett Burman design Response Surface Methodology (RSM) showed KH...