Proteases are one of the predominant groups industrial enzymes and it represents for about 65% total global enzyme market. microbial origin have great importance over plant sources because they minimize production costs, increase characteristics desired products widely used in biotechnological process. Among protease enzymes, aspartic proteases most important proteolytic which mainly produced b...

In search of plant genes expressed during early interactions between Casuarina glauca and Frankia, we have isolated and characterized a C. glauca gene that has strong homology to subtilisin-like protease gene families of several plants including the actinorhizal nodulin gene ag12 of another actinorhizal plant, Alnus glutinosa. Based on the expression pattern of cg12 in the course of nodule deve...

Objective: Investigate plant natural products with inhibitory activity of Leishmania proteases, because the inhibition certain proteases induce parasite death. Methods: Descriptive/exploratory study, integrative review type. The search was performed in following databases: Scientific Electronic Library OnLine (SciELO), Medical Literature Analysis and Retrieval System Online (MEDLINE), Latin Ame...

Pseudomonas syringae pv. tomato DC3000 causes bacterial speck disease in tomato, and it elicits the hypersensitive response (HR) in non-host plants such as Nicotiana tabacum and Nicotiana benthamiana. The compatible and incompatible interactions of DC3000 with tomato and Nicotiana spp., respectively, result in plant cell death, but the HR cell death occurs more rapidly and is associated with ef...

The distribution and subcellular localization of the two major proteases present in oat (Avena sativa L. cv Victory) leaves was investigated. Both the acidic protease, active at pH 4.5, and the neutral protease, active at pH 7.5, are soluble enzymes; a few percent of the enzyme activity was ionically bound or loosely associated with organellar structures sedimenting at 1000g. On the average, 16...

Phytocystatin, a type of protease inhibitor (PI), plays major roles in plant defense mechanisms and has been reported to show antipathogenic properties and plant stress tolerance. Recombinant plant PIs are gaining popularity as potential candidates in engineering of crop protection and in synthesizing medicine. It is therefore crucial to identify PI from novel sources like Curcuma longa as it i...

Several nonpeptide small molecules were designed as potential inhibitors of HIV protease and their structures were constructed by computer-aided molecular modeling and docked iwo the active site of HIV protease. Models of the complexes of inhibitors and the HIV protease were refined using nonbonded and H-bonding terms. The refined energy of selected complexes showed that the designed inhib...

Procerain B, a novel cysteine protease (endopeptidase) isolated from Calotropis procera belongs to Asclepiadaceae family. Purification of the enzyme, biochemical characterization and potential applications are already published by our group. Here, we report cDNA cloning, complete amino acid sequencing and molecular modeling of procerain B. The derived amino acid sequence showed high sequence ho...

Corona Virus Disease 2019 (COVID-19) caused by a novel coronavirus has spread all over the globe and become pandemic now. Until now several vaccines have come-up with limited efficacy for managing COVID-19. However, studies are still going on to find suitable therapeutic drugs which can target viral proteins or host receptor. Hence search plant-based anti-viral agents against SARS-CoV-2 is prom...