Recent reports have underlined the potential of gamma (γ)-rays as tools for seed priming, a process used in seed industry to increase seed vigor and to enhance plant tolerance to biotic/abiotic stresses. However, the impact of γ -rays on key aspects of plant metabolism still needs to be carefully evaluated. In the present study, rice seeds were challenged with different doses of γ -rays and gro...

The growth of strawberry plantlets in the rooting stage on culture medium supplemented with silver nanoparticles (AgNPs) and ethylene gas accumulation plantlet bottles were investigated. In addition, different systems first used to produce large-scale Strawberry plantlets. results showed that shoots (3 cm) cultured MS 0.02 mg/L NAA, 1 g/L activated charcoal, 30 sucrose, 8 agar 0.5 AgNPs about 4...

A complete protocol is presented for in vitro regeneration of pomegranate (Punica granatum L.), a tropical fruit tree, using cotyledonary nodes derived from axenic seedlings. Shoot development was induced from cotyledonary nodes on Murashige and Skoog (1962) (MS) medium supplemented with 2.3±23.0 mM benzyladenine (BA) or kinetin (Kn). Both type and concentration of cytokinin signi®cantly in ̄uen...

The current nitrogen fertilization for sugarcane production in Guangxi, the major sugarcane-producing area in China, is very high. We aim to reduce nitrogen fertilization and improve sugarcane production in Guangxi with the help of indigenous sugarcane-associated nitrogen-fixing bacteria. We initially obtained 196 fast-growing bacterial isolates associated with the main sugarcane cultivar ROC22...

Species of the genus Erythrina are frequently used as ornamental plants and in projects to restore degraded areas. Also, extracts from its shoots and stems are popularly used as a herbal medicine with validated activity on the central nervous system. The objective of this study was to evaluate E. mulungu and E. velutina seed germination and seedling development potential aiming commercial scale...

A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...

Polyamines (PAs) are small aliphatic polycations that are found ubiquitously in all organisms. In plants, PAs are involved in diverse biological processes such as growth, development, and stress responses. In Arabidopsis thaliana, the arginine decarboxylase enzymes (ADC1 and 2) catalyze the first step of PA biosynthesis. For a better understanding of PA biological functions, mutants in PA biosy...

An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 ...

Rapid clonal propagation through in vitro techniques using cotyledonary node as an explant has been attempted in the high yielding, tikka-susceptible (ICG 11337, AK 1224, ICGS 44, JL 24) and tikka-immune (ICG 6284) varieties of Arachis hypogaea L. Cotyledonary nodes were excised from the 12–15 days-old axenic seedlings and cultured in the presence of various concentrations of N6 –benzylaminopur...

The present investigations were attempted to develop the rapid in vitro micropropagation protocol of Moringa oleifera (Variety-PKM-1) from nodal sections of young, aseptically grown seedlings. Benzyladenine (BA) at 4.44 lM was found to be optimal in producing on maximum an average of 9.0 ± 1.0 axillary shoots per explant after 15 days of inoculation. A high multiplication rate was established t...