This study determined the plasmid profile and antibiotic resistance patterns of extended spectrum beta lactamases producing Pseudomonas aeruginosa expressing Amp C beta lactamase enzyme using standard procedures. Results obtained revealed that 32 (43.2%) had one plasmid, 37(50%) had two plasmids while the remaining five carried three plasmids each. The plasmid sizes ranged from 800bp to 25000-b...

Problem statement: The role of plasmid in the heavy metal resistance and accumulation by endophytic bacteria was investigated. Approach: The experimental results showed that high level plasmid mediated Cd and Zn resistance in this strain is due to decreased Cd and/or Zn uptake/accumulation by resistance strain. Results: Based on the fact that subsequent plasmid curing experiments demonstrated t...

Curing and genetic transfer experiments showed that lactose-fermenting ability (Lac) and the ability to produce mucoidness in milk cultures (Muc) in Streptococcus cremoris MS were coded on plasmids. The Lac phenotype was associated with a 75.8-megadalton plasmid, pSRQ2201. The Muc phenotype was associated with a 18.5-megadalton plasmid, pSRQ2202. The Lac plasmid, pSRQ2201, was first conjugative...

Plasmid transfer via the transformation pathway of Streptococcus pneumoniae was weakly restricted by the DpnI or DpnII restriction endonuclease, either of which gave a reduction only to 0.4, compared with phage infection, which was restricted to 10(-5). The greater sensitivity of plasmid transfer compared with chromosomal transformation, which was not at all restricted, can be attributed to par...

A derivative of Bacillus thuringiensis subsp. kurstaki HD1 (HD1-9) released transducing phage (TP21) from late exponential cultures. Three of seven markers tested were transduced into Bacillus cereus, but only two of these (cysC and trpB/F) were transduced at a frequency of more than 100 times the reversion rates. A limited transduction capacity was given further support in that few chromosomal...

27 Streptococcus agalactiae (group B Streptococcus, GBS) is a leading cause of sepsis in 28 neonates. The rate of invasive GBS disease in non-pregnant adults also continues to climb. 29 Aminoglycosides alone have little or no effect on GBS, but synergistic killing with penicillin 30 has been shown in vitro. High-level gentamicin resistance (HLGR) in GBS isolates, however, 31 leads to loss of a ...

Alcaligenes eutrophus A5 catabolizes biphenyl to CO2 via benzoate and 4-chlorobiphenyl to 4-chlorobenzoate. In curing and conjugation experiments, the A5 endogenous 51-kb IncP1 plasmid pSS50 was found to be dispensable for biphenyl and 4-chlorobiphenyl catabolism. Transfer of the biphenyl- and 4-chlorobiphenyl-degrading phenotype by means of pSS50 was observed at a frequency of 10(-5) per trans...

Background: The increased use of antibiotics in recent times has resulted in the development of resistance to antibiotics. The significant clinical implication of resistance has led to heightened interest in the study of bacterial resistance. Purpose: This study was carried out to evaluate the effects of antibiotic-combinations against some community isolates of multiple antibiotic-resistant St...

The idea behind the work is to check for residence of pesticide degrading genes in plasmids and transform them on an expression vector allowing degrade more efficiently. Seven different commercially used toxic pesticides were bioremediation study. Around 20 organisms first isolated based their tolerance, 11 further analysis. Each was subjected plasmid curing confirmed ability degradation plasmi...