Although the mechanism by which a kinesin-1 molecule moves individually along a microtubule is quite well-understood, the way that many kinesin-1 motor proteins bound to the same cargo move together along a microtubule is not. We identified a 60-amino-acid-long domain, termed Hinge 1, in kinesin-1 from Drosophila melanogaster that is located between the coiled coils of the neck and stalk domain...

In modern offshore installations, thin walled columns are used for energy absorption in accident situation. When this advanced offshore shock absorber is subjected to axial compressive load, plastic hinges are developed on the sides of column and the column crushes with repeatable patterns. It can absorb considerable impact energy due to large and continuous plastic deformation. This paper deve...

Blasting demolition plays an important role in building demolition, and numerical-simulation methods can facilitate the optimization of blasting-demolition schemes. To study influence delay-time differences between columns on blasting effect small-aspect-ratio reinforced concrete structures, a separated common node model structures was established through finite element numerical simulation sof...

The short 8-10 amino acid "hinge" sequence in lactose repressor (LacI), present in other LacI/GalR family members, links DNA and inducer-binding domains. Structural studies of full-length or truncated LacI-operator DNA complexes demonstrate insertion of the dimeric helical "hinge" structure at the center of the operator sequence. This association bends the DNA ∼40° and aligns flanking semi-symm...

T cells of tumor bearers often show defective TCR-mediated signaling events and, therefore, exhibit impaired immune responses. As such, patients with heavy tumor burden are often not amenable to adoptive T cell therapy. To overcome this limitation, we have developed a chimeric receptor that joins an extracellular single chain Fv (scFv) of a specific Ab for Ag recognition to an intracellular pro...