Intravenous injection of polyinosinic acid/polycytidylic acid [(poly rI).(poly rC)] offered significant protection against intranasal challenge of young mice with vesicular stomatitis virus (VSV). Optimal protection was obtained when a single dose was administered 2 hr before virus challenge, but repeated doses were effective when started as late as 3 days after virus challenge. The therapeutic...

We have examined the relationship between the newly synthesized mRNA that enters polysomes in sea urchin embryos and the messengerlike RNA that enters the pool of ribosome-free ribonucleoprotein particles (free RNPs or informosomes). Although the RNA in the free RNPs turns over 25% more rapidly than in the polysomes, labeling kinetics indicate that the RNA containing poly(A) [poly(A)(+)RNA] and...

4.1 Molecular biology methods 28 4.1.1 Construction of expression plasmids 28 4.2 Cell biology methods 29 4.2.1 Cell culture and transfection 29 4.2.2 ImmunoFISH 30 4.2.3 Microscopy, image analysis and statistical evaluation 31 4.3 Biochemical methods 32 4.3.1 In vivo protein interaction assays 32 4.3.2 In vitro protein interaction assays 33 4.3.3 Western blot analysis 34 4.3.4 In vitro poly(AD...

The thermodynamics of rightand left-handed helix formation by poly[d(G-C)].poly[d(G-C)] and by poly(dG-mSdC).poly(dG-msdC) were measured spectrophotometrically and calorimetrically. From the spectrophotometric measurements the thermal stabilities of the alternative helical conformations were evaluated as a function of counterion concentration. From the calorimetric measurements the enthalpies o...

Highly purified poly(A) polymerase (polynucleotide adenylyltransferase, EC 2.7.7.19), which synthesizes poly(A) from ATP substrate, can also catalyze hydrolysis of poly(A). The enzyme, designated as poly(A) hydrolase, requires either Mn2+ or Mg2+ for activity. Although AMP is the predominant product of the reaction, ADP and ATP are also formed. The enzyme is a 3'-exonuclease that does not degra...

Poly(pyrimidine) . poly(purine) tracts have been discovered in the 5'-flanking regions of many eucaryotic genes. They may be involved in the regulation of expression since they can be mapped to the nuclease-sensitive sites of active chromatin. We have found that poly(pyrimidine) . poly(purine) DNAs which contain 5-methylcytosine (e.g. poly[d(Tm5C)] . poly[d(GA)]) will form a triplex at a pH bel...

In the present study, a series of aliphatic polyesters based on succinic acid and several diols with 2, 4, 6, 8, 10 methylene groups, namely poly(ethylene succinate) (PESu), poly(butylene (PBSu), poly(hexylene (PHSu), poly(octylene (POSu), poly(decylene (PDeSu), were prepared via two-stage melt polycondensation method. All semicrystalline materials Tm ranging from 64.2 to 117.8 °C, while their ...

The interaction of the 1 alpha,25-dihydroxyvitamin D3 receptor with RNA and synthetic polynucleotides has been examined by using receptor from rachitic chicken intestine. Total intestinal RNA inhibited the binding of receptor to calf thymus DNA-cellulose with an efficiency equivalent to single-stranded DNA. A comparison of the inhibitory activity of several polyribonucleotides gave the followin...