Previous studies showed that the expression of connexin 32 (Cx32), the polypeptide subunit component of the major hepatic gap junction, is reduced in liver by changes in mRNA stability during bacterial lipopolysaccharide (LPS)-induced inflammation. In this study, we examined the distribution of Cx32 mRNA poly(A) tail lengths during LPS-induced inflammation, because this is considered the first ...

The reproducibility of the pull-in voltage of a single side clamped free-standing beam has been investigated for application as a DC voltage reference. A 2 mm long beam has been fabricated in an epipoly process with, at the tip, poly-poly electrodes extending into the axial direction for electrostatic lateral deflection and a combdrive operating in the normal lateral beam direction. The DC volt...

In this paper, a silicon-oxide-nitride-silicon nonvolatile memory constructed on an n+-poly-Si nanowire [NW] structure featuring a junctionless [JL] configuration is presented. The JL structure is fulfilled by employing only one in situ heavily phosphorous-doped poly-Si layer to simultaneously serve as source/drain regions and NW channels, thus greatly simplifying the manufacturing process and ...

possible that contaminants in the preparation may have contributed some of the effects observed. The present study describes the purification of MCR to a homogeneous single component as demonstrated by poly acrylamide gel electrophoresis and NH@-terminaIamino acid analysis and presents preliminary information on the ability of MCR to degrade PM2 DNA in vitro and DNA within Novikoff hepatoma cells.

The role of thymus in antibody responses to a series of four synthetic polypeptide immunogens of the general formula multi-copoly(Tyr,Glu)-poly(Pro)-poly(Lys) was investigated as a function of the optical activity of the amino acids composing their structure. Irradiated nonthymectomized and thymectomized SJL mice were injected with thymocytes, marrow cells, or a mixture of both. Each group of r...

In frog oocytes, certain maternal mRNAs receive poly(A) in the cytoplasm during progesterone-induced maturation. To analyze this reaction and to compare it to poly(A) addition in the nucleus, we injected short, synthetic RNA substrates into Xenopus oocytes. These RNAs contain only portions of the 3'-untranslated regions of appropriate mRNAs and end at the natural poly(A) site. We demonstrate th...

α active specific immunotherapy aims at stimulating the host's immune system to recognize and eradicate malignant cells. The concomitant activation of dendritic cells (DC) and natural killer (NK) cells is an attractive modality for immune-based therapies. Inducing immunogenic cell death to facilitate tumor cell recognition and phagocytosis by neighbouring immune cells is of utmost importance fo...

A method for preparing colloidal particles formed by a magnetite nucleus and a biodegradable poly(DL-lactide) polymer coating is first described. The method is based on the so-called double-emulsion technique, employed to obtain polymeric spheres loaded with therapeutic drugs, to be used as drug delivery vectors. The aim of this work was to obtain, in a reproducible and rather simple way, collo...

The spatially controlled synthesis of poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic stationary phases in polyimide coated fused silica capillaries by visible light induced radical polymerisation using a three-component initiator and a 660 nm light emitting diode (LED) as a light source is presented here.

Our proposed project is a recommendations component for web-based announcements system to be used by Cal Poly portal users. The system may hold at any time hundreds of past, present and future announcements or events pulled from disparate sources for display. To facilitate display of announcements, we will develop a recommendation system to select and sort the top announcements for a given user.