........................................................................................................................................... IX 1. GENERAL INTRODUCTION .......................................................................................................... 1 2. LITERATURE REVIEW .......................................................................................................

Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...

Background & Aims: Calcifying nanoparticles are different forms of calcium and phosphate in sediments. Recent evidence suggests that calcifying nanoparticles (CNPs) are probably selfreplicating. Several diseases are linked to nano-bacteria including kidney stones, gallbladder stone, cardiovascular plaques, oral–dental plaque, many cancers, and autoimmune diseases. The aim of this study was to a...

Utilizing ~1 W of microwave power, we achieved heating of PCR buffer with unprecedented temperature ramp rates that allowed for rapid thermocycling. Polymeric microdevices, chosen for their low cost and ease of fabrication, were constructed from polycarbonate (PC), poly(methyl-methacrylate) (PMMA), and polyester-toner (PeT) using fast, simple microfabrication via computer-aided milling for PC a...

The aim of the work is to summarize available literature data on cases visceral leishmaniasis in children registered Russian Federation, as well describe our own clinical observation autochthonous a 9-year-old child, diagnosis which polymerase chain reaction (PCR) method was used. Materials and methods: review domestic foreign over past 20 years, including Internet resources, carried out. We ob...

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Genotypes of Iranian Zel sheep for Calpastatin(CAST) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) methods and for Calpain(CAPN) locus by PCR-SSCP. Blood samples were collected from 200 purebred Zel sheep of Zel Breeding Station located in Golestan province i...