In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to...

Two types of molecular markers, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were assayed to determine the genetic diversity of 46 barley accessions, including 27 landraces of H. vulgare ssp vulgare (HV), 6 accessions of H. vulgare ssp. spontaneum (HS) and 13 accessions of H. vulgare ssp. agriocrithon (HA), from west China. A high level of polymorphism was fo...

Plantlets were regenerated through callus-mediated organogenesis after UV–B irradiation (280–320 nm, 0.863–5.28 kJ m) in Vernonia cinerea and were analyzed by Random Amplified Polymorphic DNA with 10 random decamers to assess UV–B induced polymorphism. A total of 45 different somaclones were selected based on difference in morphology compared to the parent plant. Out of the 10 primers chosen fo...

Olive grows in the mid-latitudes in the world and in areas with Mediterranean climate. Olive is one of the important agricultural products in Mediterranean Basin and Turkey. Its contribution to economy makes it valuable to determine the genetic relations among different olive varieties. In this study, genetic variations among 13 different olive cultivars were determined through 22 ISSR primers ...

Transferability of sequence-tagged-sites (STS) markers was assessed for genetic relationships study among accessions of marvel grass (Dichanthium annulatum Forsk.). In total, 17 STS primers of Stylosanthes origin were tested for their reactivity with thirty accessions of Dichanthium annulatum. Of these, 14 (82.4%) reacted and a total 106 (84 polymorphic) bands were scored. The number of bands g...

In this study, the microsatellite technique was used to evaluate the genetic variability in populations of collared and white-lipped peccaries kept in captivity. Six primers developed for domestic pigs were used and amplified in both species. They revealed the presence of five polymorphic loci and one monomorphic locus. The polymorphic loci included 4 of the 16 alleles in collared peccaries, an...

The molecular characterization of chilli germplasm was done based on estimation genetic diversity among the by using SSR markers. Forty germplasms were analyzed eight primers. primers produced 30 loci with an average value 3.75 alleles per locus. similarity index matrix ranged from zero to 2.74. Polymorphic information content (PIC) 0.543 0.735 0.658. highest number (five) allele observed in pr...

The current study was carried out with a set of 44 mulberry accessions in order to evaluate the degree distinctiveness and relatedness among selected germplasm accessions. A total 40 microsatellite markers were on basis earlier studies, which 26 primers yielded 182 polymorphic bands. PIC (polymorphism information content) values above 0.5. Based clusters obtained from unweighted pair group meth...

There is a lack of information on the molecular characterization of Ocimum species and hence, efforts have been made under the present study to characterize 17 Ocimum genotypes belonging to 5 different species (O. basilicum, O. americanum, O. sanctum, O. gratissimum and O. Polystachyon) through random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. PCR amplifi...