We studied bacterial isolates 15M and 16M from calla lily tubers showing soft rot in commercial plantations in Poland. Colony morphology differed from that of known agents of bacterial soft rot of this plant. The use of Microgen Bacillus ID system allowed to identify both isolates as Paenibacillus polymyxa. This was confirmed by sequence analysis of 16S rRNA gene fragments. The isolates showed ...

A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hype...

Bacillus anthracis causes anthrax and represents one of the most molecularly monomorphic bacteria known. We have used AFLP (amplified fragment length polymorphism) DNA markers to analyze 78 B. anthracis isolates and six related Bacillus species for molecular variation. AFLP markers are extremely sensitive to even small sequence variation, using PCR and high-resolution electrophoresis to examine...

In this study, bacterial strains were isolated from soils from 30 locations of Samcheok, Gangwon province. Of the isolated strains, seven showed potential plant growth promoting and antagonistic activities. Based on cultural and morphological characterization, and 16S rRNA gene sequencing, these strains were identified as Paenibacillus species. All seven strains produced ammonia, cellulase, hyd...

Nagel, Charles W. (University of California, Davis) and Reese H. Vaughn. Comparison of growth and pectolytic enzyme production by Bacillus polymyxa. J. Bacteriol. 83:1-5. 1962.-Studies were made of pectolytic enzyme production by Bacillus polymyxa during growth. It was found that elaboration of enzyme occurred during logarithmic growth and ceased when the stationary phase was reached. The speci...

One of the most striking characteristics of gall-forming insects is the variability in gall position, morphology, and complexity. Our knowledge of the driving forces behind the evolutionary divergence of gall types is limited. Natural enemies, competition, and behavioral constraints might be involved. We present a cladogram, based on sequences of COI and COII (1952bp), of mitochondrial DNA for ...

Evaluation of Rhizoctonia zeae as a potential biological control option for fungal root diseases of sugar beet" (2015). Abstract Several common root diseases routinely damage sugar beet in Nebraska and other production areas of the Central High Plains, and it is becoming more common to find fields infested simultaneously with multiple pathogens. Owing to the shortage of available fungicides for...

Retinoid-binding proteins play an important role in regulating transport, storage, and metabolism of vitamin A and its derivatives. The solution structure and backbone dynamics of rat cellular retinol-binding protein type I (CRBP) in the apo- and holo-form have been determined and compared using multidimensional high resolution NMR spectroscopy. The global fold of the protein is consistent with...