Bone tissue engineering offers promising alternatives to repair and restore orthopaedic disorders. Our laboratory has employed poly(lactic acid-co-glycolic acid) (PLAGA) microspheres to develop a three dimensional (3-D) porous bioresorbable scaffold with a pore structure similar to the structure of human trabecular bone. The success of osseous healing and integration of the engineered implants ...

The major protein of the outer mitochondrial membrane of Neurospora was purified. On dodecylsulfate-containing gels it displayed a single band with an apparent molecular weight of 31 000. Reconstitution experiments with artificial lipid bilayers showed that this protein forms pores. Pore conductance was dependent on the voltage across the membrane. The protein inserted into the membrane in an o...

We present spatial distributions for pore path length and coordination number, pore throat size and nodal pore volume obtained for a 1:53 mm3 volume of 12.1% porosity Fontainebleau sandstone. The sandstone was imaged using Synchrotron X-Ray computed microtomography at 6 micron resolution. The spatial distributions were computed based upon three dimensional medial axis analysis of the void space...

Lattice thermal conductivity !l of Si with nanometer-sized pores along the #001$ direction is calculated as a function of pore diameter !dp" and pore spacing !ds" by employing a molecular dynamics approach. Our results show that !l across pores is smaller than the bulk value by over two orders of magnitude at room temperature, and that it decreases !increases" as a function of dp !ds" for fixed...

Molecular Dynamics simulations of a coarse-grained bead-spring model of flexible macromolecules tethered with one end to the surface of a cylindrical pore are presented. Chain length N and grafting density σ are varied over a wide range and the crossover from “mushroom” to “brush” behavior is studied for three pore diameters. The monomer density profile and the distribution of the free chain en...

A membrane filtration technique with commercially available membrane filters (Millipore Corp.) was effective for the removal of Reiter treponemes from liquids such as fluorescent-antibody conjugates, to which the organisms are added for adsorption. Reiter treponemes from an 8-day culture were not microscopically detectable in filtrates through membranes with a pore diameter of 0.45 mum, but tre...

A new anodization method for the preparation of nanoporous anodic aluminum oxide (AAO) with pattern-addressed pore structure was developed. The approach is based on pulse anodization of aluminum employing a series of potential waves that consist of two or more different pulses with designated periods and amplitudes, and provides unique tailoring capability of the internal pore structure of anod...

Despite their great potentials as biomacromolecues delivery vehicles, there are few, if any, reports on mesoporous silica nanoparticles (MSNs) simultaneously integrated with the merits of large pore size, small particle diameters and well-ordered mesostructure. Here, we designed a facile strategy for the synthesis of monodispersed MSNs using cationic surfactants (CSs) as templating agents, neut...

The bacterial toxin pneumolysin is released as a soluble monomer that kills target cells by assembling into large oligomeric rings and forming pores in cholesterol-containing membranes. Using cryo-EM and image processing, we have determined the structures of membrane-surface bound (prepore) and inserted-pore oligomer forms, providing a direct observation of the conformational transition into th...

4 Yeast Division of the Centraalbureau voor Schimmelcultures, Julianalaan 67, 2628 BC Delft, The Netherlands The septal pore cap (SPC) of Trichosporon sporotrichoides CBS 8245 is vesiculartubular, connected with flat-tubular endoplasmic reticulum (ER), and stains densely with zinc/iodine/osmium tetroxide, as does the ER. The SPC of Schizophy//um commune CBS 340.81 is more complex, about 600 nm ...