To follow the dynamics of nuclear pore distribution in living yeast cells, we have generated fusion proteins between the green fluorescent protein (GFP) and the yeast nucleoporins Nup49p and Nup133p. In nup133- dividing cells that display a constitutive nuclear pore clustering, in vivo analysis of GFP-Nup49p localization revealed changes in the distribution of nuclear pore complex (NPC) cluster...

Transport between the nucleus and cytoplasm involves both stationary components and mobile factors acting in concert to move macromolecules through the nuclear pore complex. Multiple transport pathways requiring both unique and shared components have been identified. In the past 18 months, new findings have shed light on the nature of some of the mobile components of these pathways. New recepto...

the word parī (fairy) is the remaining of the avestan word pairīkā that derived from the indo- european root *per- 'to bear, to create', then it can be defined as 'fertile, creative'. pairīkā, in the ancient time were the admirable, beautiful and always young goddesses of birth and fertility that they were denoting to mankind fertility, birth and life with their presence. after zarathuštra made...

Nuclear pore complexes (NPCs) are enormous macromolecular structures that mediate the active exchange of proteins and RNPs between the nucleus and cytoplasm. Recent work has resulted in a windfall of identified NPC polypeptides, many with unique sequences. Several of the proteins have been shown to be part of extended cytoplasmic and nucleoplasmic NPC filaments. Biochemical, structural and gene...

FG nucleoporins (Nups) are the class of proteins that both generate the permeability barrier and mediate selective transport through the nuclear pore complex (NPC). The FG Nup family has 11 members in Saccharomyces cerevisiae, and the study of mutants lacking different FG domains has been instrumental in testing transport models. To continue analyzing the distinct functional roles of FG Nups in...

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermost...

Eukaryotic cells have developed a series of highly controlled processes of transport between the nucleus and cytoplasm. The present review focuses on the latest advances in our understanding of nucleocytoplasmic exchange of molecules in yeast, a widely studied model organism in the field. It concentrates on the role of individual proteins such as nucleoporins and karyopherins in the translocati...

The following proceeding presents a multiscale approach to investigate the hydraulic anisotropy and retention behavior in porous materials involving deformation effects. Concerning former, single-phasic fluid flow simulations using lattice Boltzmann method (LBM) are initially accomplished at pore-level geometry of Bentheimer sandstones provided by X-ray microscopy several degrees compression co...

pore pressureis defined as the pressure of the fluid inside the pore space of the formation, which is also known as the formation pressure. when the pore pressure is higher than hydrostatic pressure, it is referred to as overpressure. knowledge of this pressure is essential for cost-effective drilling, safe well planning, and efficient reservoir modeling. the main objective of this study is to ...