Glyoxalase I plays a critical role in the enzymatic defence against glycation by catalysing the isomerization of hemithioacetal, formed spontaneously from cytotoxic α-oxoaldehydes and glutathione, to (S)-α-hydroxyacylglutathione derivatives. Upon the hydrolysis of the thioesters catalysed by glyoxalase II, inert (S)-α-hydroxy acids, that is, lactic acid, are then produced. Herein, we demonstrat...

Two novel simple, precise and rapid spectrophotometric methods (Mehod A and Mehod B) are proposed for the determination of sulfamethaxazole (SFZ) using ferric chloride and potassium ferricyaide, sodium nitrite and resorcinol, as reagents. Method A is based reduction of Fe+3 by SFZ and Fe+2 produced forms complex with potassium ferricyanide to form a green colored chromogen having maximum absorp...

A new simple and sensitive kinetic spectrophotometric method has been proposed for the determination of cefadroxil in pure form and pharmaceutical formulations. The proposed method is based on the formation of yellow coloured product (λmax = 410 nm) that resulted after addition of the reagent, which is composed of a mixture of Henna extract, sodium hydroxide and potassium permanganate, to cefad...

Two simple, sensitive and economical spectrophotometric methods were developed for the determination of nifedipine in pharmaceutical formulations. Method A is based on the reaction of the nitro group of the drug with potassium hydroxide in dimethyl sulphoxide (DMSO) medium to form a coloured product, which absorbs maximally at 430 nm. Method B uses oxidation of the drug with ammonium molybdate ...

This paper studies the impact of the re-importation of imitated pharmaceuticals as a by-product of an open policy toward parallel import (PI) on process innovation. Foreign investment by a firm to exploit a new unregulated market with weak intellectual property rights can give rise to imitation. These products can potentially re-enter the original country when PI is allowed influencing research...

The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS). HFCS is used as a sweetener in food and phar...

New simple and sensitive spectrophotometric and fluorimetric methods have been developed and validated for the determination of fluoxetine hydrochloride (FLX) in its pharmaceutical formulations. The spectrophotometric method was based on the reaction of FLX with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium (pH 11) to form an orange-colored product that was measured at 490 nm. The...

A simple, sensitive and rapid ionpair high performance liquid chromatographic methodwas developed for the estimation ceftriaxone sodium (CS) and tazobactum sodium (TS) in pharmaceutical dosage forms. Lichrocart100-RP18e5μm-C18 column was used with a mobile phase containing mixture of 0.012M tetra butyl ammonium hydroxide in 0.01M potassium dihydrogen phosphate : acetonitrile in the ratio of 70:...

This paper presents a method for the determination of phenylephrine hydrochloride in pharmaceuticals by spectrophotometric flow injection analysis exploiting the reaction with potassium ferricyanide and 4-aminoantipyrine, which leads to the formation of a condensation product with strong absorptivity at 500 nm. The linear dynamic range was between 0.95 and 9 mg/L, with a limit of detection of 0...