An alignment of 5 amino acids in the Escherichia coli proline carrier (G328-A366-L371-GR376) is common in the amino acid sequences of several Na+ symport carriers, and it has been proposed as the putative sodium binding motif (Deguchi, Y., Yamato, I., and Anraku, Y. (1990) J. Biol. Chem. 265, 21704-21708). To determine whether these amino acids are essential for Na+ symport activity as the Na+ ...

BACKGROUND Glutamate dehydrogenase (GDH) is a key enzyme for the synthesis and catabolism of glutamic acid, proline and alanine, which are important osmolytes in aquatic animals. However, the response of GDH gene expression to salinity alterations has not yet been determined in macro-crustacean species. METHODOLOGY/PRINCIPAL FINDINGS GDH cDNA was isolated from Eriocheir sinensis. Then, GDH ge...

A basic protein has been isolated from pig brain. The purification procedure consisted of repeated extractions with chloroform-methanol (2: 1 v/v), 10% sodium chloride, and dilute hydrochloric acid. The pH 2.4 to 2.6 fraction obtained from the repeated acid extraction was further purified by pH and (NH&SO4 fractionation, Sephadex G-100 gel filtration, and chromatography on Amberlite IRC-50 with...

Medicinal mushrooms, including Cordyceps militaris, have received attention in Korea because of their biological activities. In the fruiting body and in corpus of C. militaris, the total free amino acid content was 69.32 mg/g and 14.03 mg/g, respectively. In the fruiting body, the most abundant amino acids were lysine, glutamic acid, proline and threonine. The fruiting body was rich in unsatura...

The effect of various proline analogs on proline oxidation in mitochondria isolated from etiolated barley (Hordeum vulgare) shoots was investigated. Of the analogs tested, only l-thiazolidine-4-carboxylic acid (T4C) was an effective inhibitor. T4C (1 millimolar) inhibited proline (10 millimolar) -dependent 0(2) uptake an average of 67%. T4C was also oxidized to some degree (12.9 nanoatoms oxyge...

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Paecilomyces tenuipes that formed on the living silkworm (Bombyx mori...

to evaluate the response of wheat to drought stress and salicylic acid, this field experiment was carried out at the experimental farm of college of agriculture, university of shahid bahonar kerman, iran in 2011-2012, in a split-plot arrangement using completely randomized block design with four replications. main plot was irrigation (normal irrigation and cut off at flowering) and sub plot was...

Since nothing appeared to be known as to the fate of proline in the animal body, it seemed desirable to make some experiments in the hope that some light might be thrown upon the changes which this amino-acid may undergo. As a first step in the investigation, observations were made on the behavior of proline when added to blood used for perfusing a surviving dog’s liver, using essentially the m...

During the course of an investigation into the biological activities and chemical properties of saliva parotin A it became necessary to study the chemical structure of this protein. Previous workers have reported that the molecular weight of saliva parotin A prepared from human saliva is 15,900 (Ito, Y. 1960). Proline, threonine, aspartic acid, and gluta mic acid are contained in saliva parotin...

The title compound [systematic name: benzoic acid-pyrrolidin-1-ium-2-carboxyl-ate (1/1)], C7H6O2·C5H9NO2, is an example of the application of non-centrosymmetric co-crystallization for the growth of a crystal containing a typically centrosymmetric component in a chiral space group. It co-crystallizes in the space group P212121 and contains benzoic acid and l-proline in equal proportions. The cr...