Plant-based diets with sufficient calcium (Ca) supplements are needed to protect the body from Ca deficiencies. The enrichment of protein ingredients during fractionation can provide a new route increase content in plant-based products. We, therefore, investigated if partial replacement NaOH by Ca(OH)2 neutralization step process affects functional properties protein-rich fractions. and oil obt...

Introduction There are many analytical tools, such as X-ray and NMR, to study protein conformation, conformation dynamics and protein-protein interactions. Each method faces different challenges and limitations depending on the protein studied. Recently, hydrogen/deuterium exchange mass spectrometry (HDX) has emerged as a powerful tool to fill the analytical gap1,2,. In this study, a fully auto...

Protein structure prediction is one of the great challenges in structural biology. The ability to accurately predict the three-dimensional structure of proteins would bring about significant scientific advances and would facilitate finding cures and treatments for many diseases. We propose a novel computational framework for protein structure prediction. The novelty of the framework lies in its...

The role that DNA conformation plays in the biochemistry of cells has been the subject of intensive research since DNA polymorphism was discovered. B-DNA has long been considered the native form of DNA in cells although alternative conformations of DNA are thought to occur transiently and along short tracts. Here, we report the first direct observation of a fully reversible en masse conformatio...

background: the expression of mouse tumor necrosis factor alpha (tnf-α) in escherichia coli is a favorable way to get high yield of protein; however, the formation of cytoplasmic inclusion bodies, which is the consequence of insoluble accumulated proteins, is a major obstacle in this system. to overcome this obstacle, we used a pulsed dilution method to convert the product to its native conform...