lysozyme, as a model protein, was precipitated through the formation of protein-zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (plga) microspheres. various parameters, including ph, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. the resulting protein parti...

Natural biopolymers from plant sources contain many impurities (e.g., fat, protein, fiber, natural pigment and endogenous enzymes), therefore, an efficient purification process is recommended to minimize these impurities and consequently improve the functional properties of the biopolymer. The main objective of the present study was to investigate the effect of different purification techniques...

Measurements with satellite and sounding rocket borne instruments contain important information on remote and local processes in regions containing matter in the plasma state. The characteristic features of the particle distributions can be used to explain the morphology and dynamics of the different plasma populations. Charged particles are lost from a region due to precipitation into the atmo...

The isolation procedure of the seed proteins of Amaranthus blitum have been analyzed at different pH conditions. Qualitative studies were carried out by using electrophoretic technique sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Mainly four protein fractions i.e. albumin, globulin, prolamin and glutelin were obtained. Protein isolates were prepared by (a) extraction a...

Ouchterlony immunodiffusion of sonicated Vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. Antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. Tandem two-dimensional immunoelectrophoretic analyses of V. vulnificus antigens from various strains ...

Bioaerosols and humic-like substances (HULIS) are important components of atmospheric aerosols, which can affect regional climate by acting as cloud condensation nuclei and some of which can damage human health. Up to date, release of bioaerosols and HULIS initiated by precipitation is still poorly understood. Here we present different release processes for bioaerosols, non-bioaerosols and HULI...

Xanthomonas citri subsp citri (Xcc) is responsible for one of the most destructive diseases of citrus fruits, Asiatic citrus canker. Based on the bacterial host range, the pathogenic forms, A, A*, and Aw have been identified. A*-type strains are not well characterized and they have been reported to differ from other A-type strains on the basis of biochemical and genetic characteristics. In this...

In this article the unique capability of elastin-like protein (ELP) to reversibly precipitate was combined with the high affinity and specificity of antibody-binding domains such as Protein G, Protein L, or Protein LG as a general method for antibody purification that combines in a unique manner the simplicity and robustness of temperature-triggered precipitation with the selectivity of affinit...