It is generally accepted that the release of thyroid hormone from its place of deposition in the follicular cavities of the gland into the systemic circulation, although under pituitary control, is directly mediated by the enzyme (or enzymes) responsible for the splitting of thyroglobulin, the storage form of the hormone. Since the cell membrane is impermeable to thyroglobulin (l), it has been ...

the substrate alone, but in the presence of the totally inactive trypsin-inhibitor complex, a re-activation of trypsin and neutral proteinase activity was observed. This can only be accounted for by the replacement of trypsin and neutral proteinase in the enzymeinhibitor complex by enzymically inactive Tos-Phe-CH,CI-chymotrypsin. Chymotrypsin and its precursor chymotrypsinogen have no proteolyt...

The thermophilic actinomycetes are the most common etiological agents causing hypersensitivity pneumonitis. Antigen preparations of these organisms contain proteolytic activity. Further investigation of the proteinases of the thermophilic actinomycetes was undertaken to determine whether this activity may contribute directly to the pathogenesis of hypersensitivity pneumonitis and pulmonary myco...

The halophilic proteinase showed a maximal activity at 50°C and pH 9~10, in 20% NaCl and was highly stabilized by NaCl. It was able to hydrolyse natural actomyosin (NAM), collagen and anchovy protein. For NAM hydrolysis, the myosin heavy chain was completely digested by halophilic proteinase as evidenced by the lowest band intensity remaining, but partially hydrolysed actin. The SR5-3 proteinas...

Infection by a baculovirus (Bombyx mori nuclear polyhedrosis virus, BmNPV) in silkworm (Bombyx mori) larvae is highly efficient as an expression system for the production of useful proteins. However, the amount of the protein of interest expressed tends to decrease in the later stages of infection presumably due, in part, to a proteinase produced in the larval haemolymph. The N-terminal amino a...

Potential resistance development to Bt cotton in certain lepidopterans has prompted research to develop strategies that will preserve this environmental-friendly biotechnology. Proteinase inhibitors are potential candidates for enhancing Bt toxicity against lepidopteran pests and for expanding the spectrum of control for other insects. Interactions of Bt toxin from Bacillus thuringiensis and pr...

The activities of citrate synthase and cytochrome c oxidase, mitochondrial marker enzymes, were evaluated in the liver of Ehrlich ascites carcinoma-bearing mice during the life span of the inoculated animals. After 10 days of tumour transplantation, when cell proliferation has ceased, a 30-40% decrease of these activities was detected in both liver and kidney. Simultaneously, an increase in the...

The production of extracellular proteinase by MY1049, a low-virulence mutant of Candida albicans, was compared with that of its wild-type parent, MY1044. Both strains were grown in a medium containing bovine serum albumin as a nitrogen source to induce proteinase production. Under these conditions, the proteinase activity per cell in the MY1049 cultures was the same as or higher than that of MY...

Semi-alkaline proteinases having optimum pH between 8 and 9 of Streptomyces origin. have been reported as products of Streptomyces erythreus,3)Streptomyces fradiae4)and Streptomyces madurae.5)The proteolytic activity of all of the above semi-alkaline proteinases can be inactivated by addition of diisopropylfluorophosphate.We have recently isolated a new semi-alkaline proteinase from the culture...