CONCLUSION: The normothermic perfusion protocol has the potential to significantly impact outcomes in limb replantation and transplantation. Our results suggest that limbs in the 12-hours perfusion group retain better physiologic parameters at the end-point. However, the continuous optimization of the protocol allowed to maintain limb’s function and preserved peripheral perfusion for up to 44 h...

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by (1)H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

This paper presents a decentralized algorithm for non-convex optimization over tree-structured networks. We assume that each node of this network can solve small-scale problems and communicate approximate value functions with its neighbors based on novel multi-sweep communication protocol. In contrast to existing parallelizable algorithms optimization, the nodes are neither synchronized nor ass...

Vehicular ad hoc network is a non infrastructure network where each network node not only acts as a host but also acts as a router. The environment is highly dynamic due to mobile nature of nodes. The proper functioning of these networks depends upon a routing protocol that can respond to the rapid changes in the topology. Our interest is focused on the OLSR routing protocol, which uses hello a...

Performance and adaptability of the transport protocol are among the most critical parts of the TCP/IP protocol stack since it is directly related to the user interaction and application experience. In this paper we analyze specific problems of reliable transport protocol performance caused by rapidly changing topology in MANETs in addition to classical transport layer challenges. Then we propo...

This paper lays down a proposal of protocol named DREAM_OLSR. The protocol has been developed so as to effect current OLSR (RFC 3626) [4] protocol. The protocol establishes an optimized solution hence the name has been manipulated from Open Link State Routing to DREAM Optimized Link State Routing. DREAM specifies Distance Routing Effective Algorithm for Mobility wherein it implements the Distan...

Recent advances in Wireless Sensor Networks (WSNs) have led to new paradigm for protocol design especially for sensor networks where energy awareness is an essential component. Most of the research, however, has been focused on to the development of routing protocols since they might differ depending on the application area and network architecture. Computational Intelligence (CI) based optimiz...

The rapid growth of heterogeneous devices and diverse networks in our daily life, makes it is very difficult, if not impossible, to build a one-size-fits-all application or protocol, which can run well in such a dynamic environment. Adaptation has been considered as a general approach to address the mismatch problem between clients and servers; however, we envision that the missing part, which ...

Seamless mobility across heterogeneous mobile wireless technologies is now essential as mobile subscribers demand full and cost-effective wireless network coverage. Under such mobility conditions the layered protocol stack is inefficient. Significant research has been done for cross layer optimizations of the protocol stack. To enable rapid deployment of existing and new cross layer optimizatio...

In this paper, we quantitatively (mathematically) reason the energy savings achieved by the Leveling and Sectoring protocol. Due to the energy constraints on the sensor nodes (in terms of supply of energy) energy awareness has become crucial in networking protocol stack. The understanding of routing protocols along with energy awareness in a network would help in energy optimization with effici...