نتایج جستجو برای: protonation constant tryptophan

تعداد نتایج: 237988  

Journal: :Journal of molecular biology 1974
V I Teichberg N Sharon J Moult A Smilansky A Yonath

Divalent copper was found to inhibit non-competitively the lysis of Micrococcus lysodeikticus cells by hen egg-white lysozyme, with an inhibition constant K, = 3.8 X lo2 M-l. The association constants of Cu2+ for lysozyme and for a derivative of lysozyme in which tryptophan residue 108 was selectively modified, were measured spectrofluorimetrically and found to be 1.8 X lo2 M-l and 1.0 X lo3 M-...

Three new rimantadine Schiff bases (RSBs) were prepared, and then the interaction of RSBs with bovine serum albumin (BSA) was investigated using fluorescence, synchronous fluorescence, UV-vis absorption spectroscopy under physiological conditions. The results showed that the three RSBs effectively quenched the intrinsic fluorescence of BSA via static quenching. Binding constant (Ka), number of ...

Three new rimantadine Schiff bases (RSBs) were prepared, and then the interaction of RSBs with bovine serum albumin (BSA) was investigated using fluorescence, synchronous fluorescence, UV-vis absorption spectroscopy under physiological conditions. The results showed that the three RSBs effectively quenched the intrinsic fluorescence of BSA via static quenching. Binding constant (Ka), number of ...

Auxin produced by plant growth-promoting bacteria (PGPR) in the wheat rhizosphere, can improve plant yield under water deficit stress condition. To investigate this issue, a completely randomized factorial design with three replications was conducted under greenhouse condition at the University of Maragheh, Eastern-Azarbayjan, Iran during 2019. Treatments were two types of growth-promoting bact...

Journal: :The Biochemical journal 1986
M E Ganapathy F H Leibach V B Mahesh J C Howard L D Devoe V Ganapathy

The characteristics of tryptophan uptake in isolated human placental brush-border membrane vesicles were investigated. Tryptophan uptake in these vesicles was predominantly Na+-independent. Uptake of tryptophan as measured with short incubations occurred exclusively by a carrier-mediated process, but significant binding of this amino acid to the membrane vesicles was observed with longer incuba...

Journal: :iranian journal of microbiology 0
marzieh dehghan-shasaltaneh department of biology, faculty of basic sciences, alzahra university, tehran, iran. zahra moosavi-nejad department of biology, faculty of basic sciences, alzahra university, tehran, iran. sara gharavi department of biology, faculty of basic sciences, alzahra university, tehran, iran. jamshid fooladi department of biology, faculty of basic sciences, alzahra university, tehran, iran.

background and objectives: the essential amino acid l-tryptophan can be produced by a condensation reaction between indole and l-serine, catalyzed by b. subtilis with tryptophan synthase activity. application of the tryptophan is widespread in the biotechnology domain and is sometimes added to feed products as a food fortifier. materials and methods: the optimum concentration of the iranian can...

Journal: :The Biochemical journal 1981
A C Aronsson S Sellin G Tibbelin B Mannervik

Glyoxalase I from human erythrocytes was studied by use of the strong reversible competitive inhibitor S-p-bromobenzylglutathione. Replacements of cobalt, manganese and magnesium for the essential zinc in the enzyme were made by a new procedure involving 10% methanol as a stabilizer of the enzyme. The K(m) value for the adduct of methylglyoxal and glutathione was essentially unchanged by the me...

Journal: :The Journal of biological chemistry 1983
J R Mattingly H A Farach M Martinez-Carrion

Two vitamin B6 derivatives, N-bromoacetylpyridoxamine (BAPM) and its phosphate ester have been found to be affinity-labeling reagents for mitochondrial aspartate aminotransferase (EC 2.6.1.1). These derivatives were first shown to react with a critical sulfhydryl group in tryptophan synthase (Higgins, W., and Miles, E. W. (1978) J. Biol. Chem. 253, 4648-4652). In the apoaminotransferase, BAPM h...

2003
HERBERT P. SARETT

Growth of animals receiving diets low in protein and nicotinic acid may be retarded by the addition of corn products (l-4), of certain amino acids (5-9), or of proteins deficient in tryptophan (8-11). This inhibition, which is obtained with diets relatively deficient in tryptophan, can be overcome by the administration of nicotinic acid or of tryptophan (l-11). It has been suggested that this d...

Objective: Interferon-α (IFN) therapy can cause depressive symptom which may lead to drug discontinuation. By interfering with tryptophan pathway, the available level of tryptophan required for serotonin synthesis decreases which could be related to depression. The aim of this study was to evaluate whether soybean diet could improve IFN-induced depression. Materials and Methods: Male mice weigh...

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