The role of actin filaments and contractions in hatching was determined in mouse blastocysts whose actin filament bundling abilities had been suppressed by H-89, an inhibitor of protein kinase A. The hatching rate of blastocysts developed from morulae in a medium containing H-89 at a concentration of 4.0 microM was 17.2%, which was significantly lower than the 76.7% of the control blastocysts d...

On $(X,\omega)$ compact K\"ahler manifold, given a model type envelope $\psi\in PSH(X,\omega)$ (i.e. singularity type) we prove that the Monge-Amp\`ere operator is an homeomorphism between set of $\psi$-relative finite energy potentials and measures endowed with their strong topologies as coarsest refinements weak such relative energies become continuous. Moreover, totally ordered family $\math...

Contraction during the blastocyst stage is observed during embryonic development of various mammals, including humans, but the physiological role of this process is not well understood. Using time-lapse monitoring (TLM), we studied the influence of vitrification and contractions on embryonic development in mice. Mouse embryos were cultured at the 2-cell stage. At the 8-cell stage, embryos were ...

The typical features of eccentric exercise-induced muscle damage is prolonged loss of muscle strength and the most rapid structural change in the fibers is loss of immunostaining for the intermediate filament protein, desmin. In this study isolated perfused rat muscle was used to examine the direct effect of temperature changes on the eccentric contraction-induced force and desmin loss. The lef...

The Agilent 1290 Infinity LC System with ultraviolet/visible (UV/VIS) Diode Array detection (DAD) is used to analyze triglycerides in soybean oil under non-aqueous reversed phase gradient conditions. The Agilent 1290 Infinity LC System was used for the chromatographic separation of the sample on 3.0 and 2.1 mm id C18 columns, of various lengths, with 1.8-μm packing materials prepared in 600 bar...