background and objectives : streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of streptococcus equisimilis strain h46a. the objective of the present study was the production of streptokinase from strain h46a and purification by chemical reduction method. materials and methods: the rate of...

background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...

Calls for medical care cost containment are all around us. Although the evidence that costs are too high is strong, the evidence that they are rising too quickly is much weaker. The principal cause of increasing costs appears to be the increased capabilities of medicine; the scant evidence available suggests that to date the public has wanted to pay for most of these capabilities. Effective glo...

The paper surveys the various economic costs associated with terrorism. Particularly important is the distinction between the direct and the indirect costs. While many terrorist attacks cause substantial amounts of property damage and create casualties, most of the costs associated with terrorism involve the indirect costs. Although terrorism can reduce a nation’s overall growth rate, the costs...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...

the rhizome of anemarrhena asphodeloides is used as food and traditional chinese medicine for its hypoglycemic effect. the aim of this study was to investigate the isolation, purification and hypoglycemic activity of anemaran as the active component. the influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. the opti...

abstract aims: one of the consequences of taking ground water into surface is changing its chemical quality, specially increasing the concentration of dissolved salts. this research was performed in order to analyze growth possibility of heterotrophic bacteria in the membrane of active desalination plants in kashan city, iran. instrument & methods: this descriptive cross-sectional study was don...