Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen sam...

Background: Egg yolk is the main cryoprotectant agent in semen freezing extenders which is used in order to protect spermatozoa against cold shock. However, elimination of animal bioproducts from the cryopreservation protocol is becoming mandatory. Therefore, the aim of this study is to compare a previously studied, homemade soya bean lecithin based extender with a commercially available extend...

The bio-stimulation technique offers a potentially useful and practical way for reproductive performance in rams. Reports on how bio-stimulation affects sexual potency rams are scarce: Knowledge of how rams respond to bio-stimulation is useful for persons who involved in semen collection; "hand" (controlled) mating, and the administration of sexual performance evaluations. The reduction of the ...

The current research was designed to compare the effects of substitution egg yolk (EY) in ram extender with Arabic gum (AG) different concentration cooled or frozen semen collected by artificial vagina (AV) electro-ejaculator (EE) Noemi rams. Semen 2 times per week for 6 weeks from rams EE method and after a rest period weeks, samples were AV method. Tris either contained 15% EY (Control) 3%, 5...

Clusterin is a major protein in bull reproductive tract secretions and sperm membrane extract. A polyclonal antibody was produced against clusterin from bull cauda epididymal fluid (CEF) and used for the localization of the protein on bull spermatozoa. Immunoblotting of unreduced bovine samples showed that the anticlusterin antibody reacted with a protein of approximately 94- to 100-kd in rete ...

Antibiotics are added to semen extenders to take care of heavy microbial load, however, their continuous use poses a constant threat of developing antibiotic resistance by the common microbes present in the semen. Our hypothesis was that natural honey, having antibacterial activity and rich in fructose could replace the use of antibiotics and fructose in the semen extender. Twenty-four ejaculat...