CircRNAs have become a novel scientific research hotspot, and an increasing number of studies shed light on their involvement in malignant progression. Prompted by the apparent gap circRNAs from apoptosis-related genes, such as BOK, we focused identification BOK human ovarian prostate cancer cells. Total RNA was extracted cell lines reversely transcribed using random hexamer primers. A series P...

Background Ziziphi spinosae semen (ZSS), dry mature seeds of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H.F. Chow., are widely used in the treatment insomnia, fright palpitations, and profuse dreaming medical treatments. The counterfeit species ZSS, mauritianae (ZMS), Hovenia dulcis Thunb (HDT) from incurva Roxb. acerba Lindl., respectively. However, pharmacopeia is not recorded for ZMS H...

Random amplified microsatellite polymorphism (RAMP) is a PCR-based marker which uses a combination of two classes of markers: Simple sequence repeat (SSR) and Random amplified DNA polymorphism (RAPD) markers. RAMP has been demonstrated to be a potentially valuable molecular marker for the study of genetic relationships in cultivated plant species. The objective of this study was to optimize the...

Reverse transcription of RNA is an invaluable method for gene expression analysis by real-time PCR or microarray methods. Random primers of varying lengths were compared with respect to their efficiency of priming reverse transcription reactions. The results showed that 15-nucleotide-long random oligonucleotides (pentadecamers) consistently yielded at least 2-fold as much cDNA as did random hex...

in recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of the environmental contaminants. in this study, the root length and dna band pattern of root tips in maize (zea mays l.) seedlings were exposed at different concentration of cadmium pollutant (40 and 80 mg l−1). the results indicated that the root length of ma...

Random-amplified-polymorphic-DNA(RAPD) was assayed to detect the genetic variation of 6 barley generations from Iraq. Four primers generated a total 17 scoreable bands in RAPD analysis) and resolving power, three polymorphic differed (Rp). The use marker systems distance among generation discovered be beneficial. dendrograms indicate diverse grouping barely specimens, although we did see that c...

wild grasses are the most important primary feedstuffs which are susceptible to contamination with toxigenic fungi belonging to aspergillus spp. in order to explore diversity of aspergillus species associated with the inflorescences of gramineous weeds, infected inflorescences were collected from wild grasses in western parts of iran. fifty-six aspergillus isolates were obtained from all diseas...