The translocation between chromosomes 15 and 17 t(15;17) at the promyelocytic leukemia (PML) retinoic acid receptor alpha (RARA) is thought to be specific for acute (APL). We present a case of with evidence by banding karyotype, FISH, PCR, rare microgranular promyelocytes significant basophilia detected on marrow aspirate, an ETV6 missense mutation molecular diagnostic testing. patient underwen...

Acute promyelocytic leukemia (APL) is an interesting model for cancer research because of the presence of the specific PML-RARa fusion gene associated with the clinical response t o retinoic acid differentiation therapy. To better understand and improve differentiation induction with retinoic acid, we have established a human APL-ascites model in SCID mice using the NB4 human APL cell line. NB4...

We present a rare case of microgranular variant acute promyelocytic leukemia (APL) associated with ider(17)(q10)t(15;17)(q22;q12) of an old-age patient. The initial chromosome study showed a 46,XX,del(6)(?q21q25),der(15)t(15;17)(q22;q12),ider(17)(q10)t(15;17)[10]/47,sl,+ider(17)(q10)t(15;17)[3]/46,XX[16]. FISH signals from a dual color dual fusion translocation PML-RARA probe were consistent wi...

Many tumors are believed to bemaintained by a small number of cancer stem–like cells, where cure is thought to require eradication of this cell population. In this study, we investigated the dynamics of acute promyelocytic leukemia (APL) before and during therapy with regard to disease initiation, progression, and therapeutic response. This investigation used a mathematical model of hematopoies...

On May 2012 issue of Leukemia, Valleron et al. presented a specific gene-expression signature, characterizing acute promyelocytic leukemia (APL), which was recognizable by ectopic expression of SNORD112–114 small nucleolar RNAs (snoRNAs) located at the DLK1-DIO3 locus. These findings were obtained using microarrays and a high-throughput quantitative PCR strategy. In their in vitro experiments c...

Preliminary Acute Promyelocytic Leukemia (APL) whole exome sequencing (WES) studies have identified a huge number of somatic mutations affecting more than a hundred different genes mainly in a non-recurrent manner, suggesting that APL is a heterogeneous disease with secondary relevant changes not yet defined. To extend our knowledge of subtle genetic alterations involved in APL that might coope...

Structurally and functionally altered retinoic acid receptors have been associated with rare human neoplasms: acute promyelocytic leukemia and hepatoceilular carcinoma. Whereas the retinoic acid receptor 13(RAR13) rearrangement in hepatocellular carcinoma is unique, in acute promyelocytic leukemia (APL), RARU fusion to the promyelocytic leukemia (PML) gene by the t(15;17) translocation is a gen...

Fourteen patients with PML/RARa-positive acute promyelocytic leukemia (APL) were given salvage therapy at the time of first molecular relapse. All patients had achieved first molecular remission after the AIDA protocol (all-trans retinoic acid [ATRA] 1 idarubicin) and were being prospectively monitored by reverse transcriptase-polymerase chain reaction (RT-PCR). Molecular relapse was defined as...

Etoposide and other topoisomerase II-targeted drugs are important anticancer therapeutics. Unfortunately, the safe usage of these agents is limited by their indiscriminate induction of topoisomerase II-mediated DNA cleavage throughout the genome and by a lack of specificity toward cancer cells. Therefore, as a first step toward constraining the distribution of etoposide-induced DNA cleavage sit...