OBJECTIVE The main purpose of this study is to emphasize the relevance of exfoliative cytology as an additional tool to aid in the diagnosis of diabetes mellitus. MATERIALS & METHODS This is a comparative cross-sectional study. Oral smears were obtained from 10 diabetic patients and 10 healthy individuals. These smears were stained with Papanicolaou stain. The nuclear (NA) and cytoplasmic (CA...

Cold acclimation dramatically increases freezing tolerance in many temperate plant species. An understanding of cold acclimation is important for extending adaptation areas of perennial ryegrass. Freezing tolerance is greatly increased in perennial ryegrass cv. 'Caddyshack' after cold acclimation. Genes differentially regulated during cold acclimation were identified by analyzing the abundance ...

the data obtained from field and geothermometric studies and also the ree composition of fluorites associated with lead mineralization at the kamar-mehdi mine are compatible with a structurally controlled, fissure filling, epigenetic, hydrothermal origin. homogenization and last ice melting temperatures for primary fluid inclusions trapped in fluorite crystals indicate that the mineralization o...

An experiment was conducted to investigate the effects of K/Ca ratios of nutrient solution on physiological traits, cut flower vase life and postharvest changes of rose. The main experiment was factorial based on completely randomized design with two cvs. of rose (Magic Red and Capitol), five K/Ca ratios in the nutrient solution (4:4, 6:4, 6:3, 8:3, 10:2) and three replications. Increasing the ...

This study examined the mechanism by which amiloride dissociates Na and Ca transport in distal convoluted tubules. Control rates of Na uptake averaged 288 nmol/(min mg protein) and were inhibited 39% by microM amiloride. Amiloride had no effect on Cl uptake. Resting membrane voltage, measured with the voltage-sensitive dye DiOC6 (3), averaged -70 mV. Amiloride hyperpolarized cells in a reversib...

Although inhibition of the sarcolemmal (SL) Na(+)-K(+)-ATPase is known to cause an increase in the intracellular concentration of Ca(2+) ([Ca(2+)](i)) by stimulating the SL Na(+)/Ca(2+) exchanger (NCX), the involvement of other SL sites in inducing this increase in [Ca(2+)](i) is not fully understood. Isolated rat cardiomyocytes were treated with or without different agents that modify Ca(2+) m...

Waxapple (Syzygium samarngense Merr.et Perry) is an important tropical fruit in Taiwan. The famous producing area is located on the coast in Pingtung County. Land subsidence and climate change will tend to soil alkalization more seriously. This study was to evaluate the effects of NaCl in waxapple seedlings. NaCl salinity reduced waxapple shoot growth; it may due to reducing relative water cont...

Using highly purified sarcolemmal vesicles isolated from dog ventricles, we examined the effects of pH on Na-Ca exchange. The initial rate of Nai-dependent Ca uptake is a sigmoid function of pH. The Ca uptake is inhibited at pH 6 and stimulated at pH 9 (as compared with uptake at pH 7.4). This dependence on pH suggests that the ionization state of a histidine residue may be important in Na-Ca e...

The relative contribution of the sarcoplasmic reticulum (SR), the L-type Ca(2+) channel and the Na(+)/Ca(2+) exchanger (NCX) were assessed in turtle ventricular myocytes using epifluorescent microscopy and electrophysiology. Confocal microscopy images of turtle myocytes revealed spindle-shaped cells, which lacked T-tubules and had a large surface area-to-volume ratio. Myocytes loaded with the f...

In this study, we test the hypothesis that in newborn hearts (as in adults) hypoxia and acidification stimulate increased Na(+) uptake, in part via pH-regulatory Na(+)/H(+) exchange. Resulting increases in intracellular Na(+) (Na(i)) alter the force driving the Na(+)/Ca(2+) exchanger and lead to increased intracellular Ca(2+). NMR spectroscopy measured Na(i) and cytosolic Ca(2+) concentration (...