We investigated the effects of two antimicrobial peptides (AMPs) isolated from Scolopendra subspinipes mutilans on neutrophil activity. Stimulation of mouse neutrophils with the two AMPs elicited chemotactic migration of the cells in a pertussis toxin-sensitive manner. The two AMPs also stimulated activation of ERK and Akt, which contribute to chemotactic migration of neutrophils. We found that...

The surface expression CD63 antigen in rat basophilic leukemia cells (RBL-2H3) was observed after antigen stimulation by confocal fluorescence microscopy. The surface expression of CD63 antigen reflected the degranulation in RBL-2H3 cells. Then, we did the same experiments in P815 mastocytoma cells with transfected IgE receptors. The expression was observed in P815 cells with normal IgE recepto...

Exhaled nitric oxide (eNO) is decreased by cigarette smoking. The hypothesis that oxides of nitrogen (NOX) in cigarette smoke solution (CSS) may exert a negative feedback mechanism upon NO release from epithelial (AEC, A549, and NHTBE) and basophilic cells (RBL-2H3) was tested in vitro. CSS inhibited both NO production and degranulation (measured as release of beta-hexosaminidase) in a dose-dep...

Wir Lehrenden lassen die Studierenden mit dem Lernen oft allein, wir halten es für ihre originäre, eigene Angelegenheit – und messen anschließend mit der Strenge einer Prüfung den Erfolg ihrer Lernbemühungen. Die Idee der lernzentrierten Lehre stellt das Lernen in den Mittelpunkt des Interesses. Lernzentrierte Lehre bereitet systematisch auf die Prüfungssituation vor und gibt den Studierenden L...

Abstract Background Coronavirus disease 2019 (COVID-19) emerged in China December 2019. The COVID-19 pandemic hindered dental education, as school buildings were closed. Online teaching provided an alternative tool for education. However, the efficiency of online and student preferences are unclear. Aim To investigate satisfaction with practices among undergraduate students standardized residen...

The synthesis and biological evaluation of a series of novel norlignans are described. Norlignans were evaluated for their inhibitory activity on the release of β-hexosaminidase, a marker of degranulation, from RBL-2H3 cells induced by the IgE-antigen complex. The results showed that norlignans 4c and 4e potently inhibited degranulation, with IC(50) values of 18.3 and 17.9 μM, respectively.

tissues including the skin, intestine, lung, heart, and dura mater in which actual membrane–membrane contacts were formed. Functional communication between mast cells and nerves has been shown to occur in physiological and pathological situations. In fact, psychological stress appears to activate mast cells and can exacerbate inflammatory responses. Dermal contacts between mast cells and nerves...

Diabetes mellitus is a disease related to hyperglycemia and insulin resistance that can lead the outcome of chronic liver diseases such as nonalcoholic fatty (NAFLD). Red betel leaves are known traditional plants have anti-hyperglycemic potential. This study aimed investigate effect ethanolic extract red nanoparticle (RbL-Nps) on hepatic receptor’s (INSR) expression in diabetic rats. Thirty rat...

Background Calculating radiographic bone loss (RBL) can be time-consuming, labor-intensive, and examiner dependent. Artificial intelligence (AI) models have been developed to automate the detection of RBL risk developing periodontal disease tooth loss. The aim this scoping review was identify evaluate performance AI in diagnosing by detecting RBL.Types Studies Reviewed Medline, Embase, Web Scie...

The CC chemokine receptor CCR5 mediates chemotaxis of leukocytes and serves as a principal co-receptor for macrophage-tropic human immunodeficiency virus type 1. To identify determinants on the CCR5 carboxyl-terminal domain that regulate receptor signaling and internalization, we generated several CCR5 mutants, which were progressively shortened from the COOH terminus or had carboxyl-terminal s...