The Polymerase Chain Reaction (PCR) has facilitated the detection of unculturable microorganisms in virtually any environmental source and has thus been used extensively in the assessment of environmental microbial diversity. This technique relies on the assumption that the gene sequences present in the environment are complementary to the "universal" primers used in their amplification. The re...

Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-specific gene insertion at desired genomic loci. However, a lack of consensus exists regarding a perfect genomic safe harbour (GSH) that would...

Increased human activities and environmental changes may lead to genetic diversity variations of Cladocerans in water. Daphnia pulex are distributed throughout the world and often regarded as a model organism. The 16S rDNA, cytochrome c oxidase subunit I (COI), and 18S genes were used as molecular marks. The genetic diversity and phylogeny of D. pulex obtained from 10 water bodies in the middle...

Molecular methods are important tools in the diagnosis of bloodstream bacterial infections, in particular in patients treated with antimicrobial therapy, due to their quick turn-around time. Here we describe a new broad-range real-time PCR targeting the 23S rDNA gene and capable to detect as low as 10 plasmid copies per reaction of targeted bacterial 23S rDNA gene. Two commercially available DN...

We have examined the structure of nuclear genes coding for ribosomal RNAs in maize and its wild relatives, the teosintes and Tripsacum. Digestion of the rDNA (genes coding for 18S, 5.8S and 26S RNAs) with 15 restriction endonucleases (with six base pair recognition sites) yields essentially a single map for the approximately 10,000 repeat units within an individual plant or species. Both length...

The classification of Trachelomonas and Strombomonas is based on the morphology loricae, which may not reflect phylogenetic relationships. There are different views relationship between two genera. It thus important for researchers to classify genera more characteristics besides such as protoplast molecular data, establish a natural system. In this study, 29 strains (13 species) 12 (eight were ...

Deuterodon is a genus of the subfamily Stethaprioninae, group Neotropical fish known as tetras. hastatus represents species complex, which supported by cytogenetic and molecular data. In this study, we show results comparative evolutionary analyses ATP synthase subunit 6 gene in four species, addition to ribosomal markers (18S rDNA 5S rDNA), new population D. complex from Angra dos Reis/RJ regi...

introduction and aims: until today human stomach is the only recognized habitat of h. pylori . however, recruitment of dna-based methods has made possible the detection of h. pylori in water and oral cavity, thus suggesting faecal-oral and oral-oral routes for transmission of h. pylori , respectively. in this study yeast has been proposed as a common vector for transmission of h. pylori . thus ...