It has been demonstrated that the in vitro assembly of microtubules from Chlamydomonas preparations does not occur under a wide range of conditions, including those efficacious for mammalian brain tubulin. This incompetence of Chlamydomonas extracts to form microtubules is independent of the tubulin concentration, the presence of added nucleotides or an added seed, temperature, or the concentra...

One of the great mysteries of the nucleolus surrounds its disappearance during mitosis and subsequent reassembly at late mitosis. Here, the relative dynamics of nucleolar disassembly and reformation were dissected using quantitative 4D microscopy with fluorescent protein-tagged proteins in human stable cell lines. The data provide a novel insight into the fates of the three distinct nucleolar s...

We describe a computational model of DNA shuffling based on the thermodynamics and kinetics of this process. The model independently tracks a representative ensemble of DNA molecules and records their states at every stage of a shuffling reaction. These data can subsequently be analyzed to yield information on any relevant metric, including reassembly efficiency, crossover number, type and dist...

Reconstruction of broken fragments is an extremely difficult task, mainly due to the high probability of missing pieces and the likelihood of erosion. This process is tedious and requires many hours to complete even for the experienced archaeologist. This paper describes some of the most successful fragment reconstruction algorithms and presents a new technique for reassembly. The technique rel...

NEM prevents mitotic reassembly of Golgi cisternae into stacked structures. The major target of NEM is a 65 kDa protein conserved from yeast to mammals. Antibodies to this protein and a recombinant form of it block cisternal stacking in a cell-free system, justifying its designation as a Golgi ReAssembly Stacking Protein (GRASP65). One of the two minor targets of NEM is GM130, previously implic...

The deployment of connectionless servers is one potential method of providing datagram service over the connection oriented ATM networks. The performance of connectionless servers are heavily in uenced by two factors. The rst factor is the packet forwarding mode. A reassembly mode connectionless server reassembles datagrams from their constituent cells before routing and forwarding them to the ...