The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide path...

A high speed and reduced-area 2-D discrete wavelet transform (2-D DWT) architecture is proposed and the design is simulated. Initially modifications are made to the lifting scheme, and the intermediate results are recombined and stored to reduce the number of pipelining stages. The architecture uses three basic 1D-DWT image processing elements. The proposed architecture only requires less regis...

FindFlow is a search interface that enables users to construct queries visually on the screen. Because the constructed queries show the process of the search, the user can take a step forward in the search while monitoring the process. The queries can be recombined freely, and previous search results can be reused. In addition, FindFlow shows results interactively for each operation. FindFlow i...

The site of action of the mutation fuzzy (fz) was investigated by the method of dermalepidermal recombination. Skins from 14-day mutant and normal mouse embryos were separated into dermal and epidermal components, recombined, and grown in the mouse testis for 14 days. Grafts that contained mutant epidermis as one of the components produced many abnormal hairs characteristic of fuzzy mice. There...

Examination of the sequences of the first complementarity-determining segments of the light chains of two IgM cold agglutinins agains blood group I, four monoclonal IgM antibodies against IgG proteins, and of three Bence Jones proteins provides clues for predicting which residues contribute to antibody specificity and indicates that these predictions may be tested by evaluating recovery of anti...