Oblique plane microscopy (OPM) enables high speed, volumetric fluorescence imaging through a single-objective geometry. While these advantages have positioned OPM as valuable tool to probe biological questions in animal models, its potential for vivo human is largely unexplored due typical use with exogenous fluorescent dyes. Here we introduce scattering-contrast oblique microscope (sOPM) and d...

Abstract The Lewis blood group system is unique because antigens are neither alleles of the same gene nor they synthesized by red cells (RBCs); rather, adsorbed onto RBC membrane from plasma as glycolipids. Antibodies against predominantly naturally occurring immunoglobulin (Ig)M type that sometimes react at 37°C and antihuman globulin phase. compound antigens, ALe b BLe , have been described w...

The mercury concentration in red blood cells (RBCs) is one of the most useful indicators for people exposed to methylmercury. Sex- and age-related differences in mercury concentrations in red blood cells were studied by considering fish consumption in 1,253 adults (511 males and 742 females) participating in a mass health examination conducted in a town of Kumamoto Prefecture in 1989. The resul...

Alloimmune feto-maternal destruction of blood cells is thought to be mediated by binding of alloantibodies to Fc receptors on effector cells. Blocking the antigen using inert antibodies might prolong cell survival. We have performed a "proof of principle" study in volunteers to measure the intravascular survival of autologous red cells coated with human recombinant IgG antibody containing a nov...

Blood transfusions are regarded as the most well-known and frequently performed cell transplantations. Although current transfusion systems sophisticated, they cannot be freed from inherent difficulties that include infection, short shelf life, blood type mismatching. Artificial oxygen carriers produced using hemoglobin (Hb) designated Hb-based (HBOCs), which anticipated for use biomaterials ha...

Blood is a complex biological fluid composed of deformable cells and platelets suspended in plasma, a protein-rich liquid. The peculiar nature of blood needs to be considered when designing a drug delivery strategy based on systemically administered carriers. Here, we report on an in vitro fluid dynamic investigation of the influence of the microcapillary flow of red blood cells (RBCs) on micro...

To date, the use of red blood cells (RBCs) produced from stem cells in vitro has not proved practical for routine transfusion. However, the perpetual and widespread shortage of blood products, problems related to transfusion-transmitted infections, and new emerging pathogens elicit an increasing demand for artificial blood. Worldwide efforts to achieve the goal of RBC production through stem ce...

Red blood cells (RBCs) degrade progressively during the weeks of refrigerated storage. No universally accepted definition of 'fresh' or 'old' RBCs exists. While practices vary from country to country, preservative solutions permitting shelf life as long as 7 weeks have been licenced. Transfusion of stored RBCs, particularly those at the end of the approved shelf life, has been implicated in adv...

Under physiologic conditions a small percentage ( 3%) of red blood cells (RBCs) are irreversibly trapped within the fibrin clot mesh. By contrast, in prothrombotic states such as diabetes mellitus, significantly more RBCs are tightly bound to fibrin fibers. Such interactions affect clot properties, making it more resistant to fibrinolytic degradation. This phenomenon may explain thrombolytic re...

This review article provides a critical survey of work from 1904 to 2003 on the effects low ionic strength in Red Blood Cells (RBCs) incubated media with impermeable sugars such as sucrose. In Gürber A washed RBCs different species isotonic sucrose solution eliminate outside ions order better analyse their intracellular composition; however, this approach was not feasible because substantial sa...