A two-step procedure was found to be useful for the efficient refolding of a complex protein, ovotransferrin. In the first step, the reduced and denatured form of the protein was incubated at a low temperature in a nondenaturing buffer containing reduced glutathione; in the second step, the reduced form was reoxidized at a higher temperature in the presence of oxidized glutathione. Under these ...

The restoration of the degenerated articular cartilage in patients with osteoarthritis (OA) is still a challenge for researchers and clinicians. Drug interventions and surgical treatments have been widely attempted for cartilage regeneration in OA. However, the results were largely unsatisfactory. Autologous chondrocyte implantation (ACI) or matrix-induced autologous chondrocyte implantation (M...

The hydroxylation of n-alkanes, which proceeds in the presence of a P450-monooxygenase advantageously at temperatures significantly below room temperature, is described. In addition, an enzymatic hydroxylation of the "liquid gas" n-butane with in situ cofactor regeneration, which does not require high-pressure conditions, was developed. The resulting 2-butanol was obtained as the only regioisom...

Freshwater planarians have remarkable regeneration ability. We did the elementary experiment which utilized the regeneration capacity of this planarian for safety evaluation of chemicals. The regeneration level of planarian measured the regeneration rate from the tail side where the reproduction of eyes was able to watch. This time, we examined the regeneration inhibition of the planarian by th...

To decrease the operating cost of flue gas purification technologies based on carbon-based materials, the adsorption and regeneration performance of low-price semi-coke and activated coke were compared for SO2 and NO removal in a simulated flue gas. The functional groups of the two adsorbents before and after regeneration were characterized by a Fourier transform infrared (FTIR) spectrometer, a...

The deactivation of Au/CeO2 catalysts during low-temperature water–gas shift reaction and the underlying physical origin were investigated, comparing the deactivation in different reaction atmospheres and subsequent regeneration of the catalysts by reactive treatment in O2/N2 or H2O/N2 gas mixtures. Based on TEM and XRD measurements, Au and ceria particle sintering (irreversible deactivation) c...

the effects of three levels of transmembrane pressure (1.0, 1.5 and 2.0 mpa) as well as three temperature  levels of: 30, 40 and 50°c on the efficiency of nanofiltration process of waste brine from the regeneration of ion exchange resins at sugar industry (to recover water and sodium chloride) were investigated throughout the current study. the polyamide tubular afc40 membrane manufactured by p...