There is increasing awareness that exposure to endocrine disrupters interferes with lipid homeostasis in vertebrates, including fish. Many of these compounds exert their action by binding nuclear receptors, such as peroxisome proliferator-activated receptors and retinoid X receptor. This work investigates the use fish liver cells (PLHC-1 ZFL cells) for screening metabolic aquatic environment as...

A three-step purification has been developed for the cellular retinoland cellular retinoic acid-binding proteins from extracts of bovine retina. The two binding proteins elute together in Step 1 (gel filtration) and separate in Step 2 (DEAE-cellulose chromatography), and each is purified to apparent homogeneity in the third step (calcium phosphate gel chromatography). The retinoland retinoic ac...

A three-step purification has been developed for the cellular retinoland cellular retinoic acid-binding proteins from extracts of bovine retina. The two binding proteins elute together in Step 1 (gel filtration) and separate in Step 2 (DEAE-cellulose chromatography), and each is purified to apparent homogeneity in the third step (calcium phosphate gel chromatography). The retinoland retinoic ac...

Retinoic acid is known to cause the cell cycle arrest and myeloid differentiation of HL-60 myeloblastic leukemia cells. Evidence suggesting the possible involvement of the Fc RII immunoglobulin receptor in mediating retinoic acid-induced growth arrest and differentiation of HL-60 cells is presented. HL-60 cells stably transfected with the 205 mutant polyoma middle T antigen, a largely debilitat...

Liarozole is a new imidazole derivative with antitumoral properties. Effects of the compound alone and in combination with all-trans-retinoic acid on proliferation of MCF-7 human breast cancer cells were examined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay. Following 9 days of drug exposure, MCF-7 cell growth was concentration dependently inhibited by all-tran...

Retinoic acid receptor (RAR) and retinoid X receptor are ligand-induced transcription factors that belong to the nuclear receptor family. The receptors are activated by small hydrophobic compounds, such as all-trans-retinoic acid and 9-cis-retinoic acid, respectively. Interestingly, these receptors are also targets for a number of exogenous compounds. In this study, we characterized the biologi...

The purpose of this study was to investigate whether and to what extent the steric isomerization of retinoic acids in conceptal tissues can be attributed to enzymatic catalysis in addition to thiol-dependent, nonenzymatic catalysis. Conversions of 13-cis-retinoic acid and 9-cis-retinoic acid to all-trans-retinoic acid catalyzed by cell-free preparations of conceptal rat tissues (gestational day...

Previous studies in our laboratory showed a synergistic interaction of synthetic ligands selective for the retinoid receptors RAR and RXR in regard to teratogenic effects produced in mice (M. M. Elmazar et al., 2001, TOXICOL: Appl. Pharmacol. 170, 2-9). In the present study the influence of phytol and phytanic acid (a RXR-selective ligand) on the teratogenicity of retinol and the RAR-selective ...

introduction: retinoid signaling has been argued to have favorable effects on alzheimer's disease (ad). we studied the role of chronic intracerebroventricular (icv) injection of all-trans retinoic acid (atra) on the amyloid-beta (aβ) model of ad. methods: adult male rats weighing 260-330 g were divided into 12 groups of 8 each. six groups of rats received atra (3nm, 30nm, 3μm, 0.3mm, 30mm/...