objective(s): the effect of litter size and suckling intensity on the expression of kiss-1 mrna in the arcuate nucleus (arc) of rats were evaluated. materials and methods: thirty two pregnant and four non-lactating ovariectomized (as control group) rats were used in this experiment. lactating rats were allotted to eight equal groups. in three groups, litter size was adjusted to 5, 10, or 15 pup...

this study was conducted to optimize a highly efficient mrna transfection into dendritic cells (dc) derived from esophageal squamous cell carcinoma (escc) patients. applying an electroporation technique, in vitro synthesized green fluorescent protein (gfp) mrna was transfected as an indicator into the dcs derived from a healthy donor. flow cytometry revealed 84.9% transfection efficiency for dc...

Background: Maternal mRNA degradation is a selective process that occurs in waves corresponding to important developmental transitions such as resumption of meiosis, fertilization and zygotic genome activation. It has been demonstrated that the number, position, and combination of 3 UTR cis-acting elements interacting with trans-acting protein factors regulate translation and mRNA stability. Ou...

Protein synthesis is one of the most complex cellular processes, involving numerous translation components that interact in multiple sequential steps. The most complex stage in protein synthesis is the initiation process. The basal set of factors required for translation initiation has been determined, and biochemical, genetic, and structural studies are now beginning to reveal details of their...

A large-eddy simulation (LES) of a bluff-body-stabilized flame has been carried out using a new strategy for LES grid generation. The recursive filter-refinement procedure (RFRP) has been used to generate optimized clustering for variable density combustion simulations. A methane–hydrogen fuel-based bluff-body-stabilized experimental configuration has been simulated using state-of-the-art LES a...

Introduction: Reactive oxygen species (ROS) have been suggested to play an important role in the myocardial damage induced by ischemia – reperfusion. One element believed to be activated by ROS and to contribute to the reduction of ROS production, is the uncoupling protein-2 (UCP2). The objective of this investigation was to explore the effect of myocardial ischemia reperfusion on cardiac UC...

Swissmedic und die Europäische Arzneimittelagentur informierten, dass Peri-/Myokarditiden nach Applikation der Messenger-RNA-(mRNA-)COVID-19-Impfstoffe Tozinameran (Pfizer/­BioNtech) mRNA-1273 (Moderna) auftreten können.