objectives: the presence of e.coli in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. the objective of this study was to develop an improved pcr method based on species – specific 16 s rrna gene primers (fes, res) for detection o...

identification  of clinically  significant  nocardia  species  is essential  for the definitive diagnosis, predict antimicrobial susceptibility, epidemiological purposes,  and  for  an  effective  treatment.   conventional   identification   of nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic...

rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...

Background and Aims: Hepatitis C virus is one of the viral infections which is mainly transmitted by blood transfusion. Patients with thalassaemia frequently need blood transfusion and are in danger of HCV infection. In most cases of infection (85%) the virus evades the immune system and establishes a chronic infection that may lead to cirrhosis and liver carcinoma. Liver is the main site of HC...

هدف: همان‌گونه که مشهود است، در سازمان‌های خدماتی به‌منظور برندسازی نیاز است ارزش‌های برندشان فعال و نهادینه شود؛ با این پیش‌فرض، پژوهش پیش‌رو هدف استخراج مدل فعال‌سازی برند داخلی از منظر فعالیت‌های مدیریت منابع انسانی تعریف اجرا شده است.طراحی/ روش‌شناسی/ رویکرد: روش مطالعۀ موردی رویکرد کیفی بانک ملت برای توسعۀ پیش گرفته شد. دو مرحلۀ اصلی طراحی گردید مصاحبه‌های عمیق فردی ۱۹ مدیر کارشناس مربوطه ...

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...

Die RNA ist ein Molekül, das sowohl genetische Informationen speichern als auch katalytische Reaktionen ausführen kann. Dieser beobachtete Dualismus rückt die ins Rampenlicht der Konzepte über den Ursprung des Lebens. RNA-Welt-Hypothese geht davon aus, dass Leben aus selbst replizierenden RNA-Molekülen entstand, sich zu immer komplexeren Strukturen entwickelten. Vor kurzem haben wir gezeigt, mi...