Regulation of SMN2 exon 7 splicing is crucial for the production of active SMN protein and the survival of Spinal Muscular Atrophy (SMA) patients. One of the most efficient activators of exon 7 inclusion is hnRNP G, which is recruited to the exon by Tra2-β1. We report that in addition to the C-terminal region of hnRNP G, the RNA Recognition Motif (RRM) and the middle part of the protein contain...

The mixed lineage leukemia (MLL) gene plays a critical role in epigenetic regulation of gene expression and is a frequent target of chromosomal translocations leading to leukemia. MLL plant homeodomain 3 (PHD3) is lost in all MLL translocation products, and reinsertion of PHD3 into MLL fusion proteins abrogates their transforming activity. PHD3 has been shown to interact with the RNA-recognitio...

Submodular maximization has attracted extensive attention due to its numerous applications in machine learning and artificial intelligence. Many real-world problems require maximizing multiple submodular objective functions at the same time. In such cases, a common approach is select representative subset of Pareto optimal solutions with different trade-offs among objectives. To this end, paper...

Background: In angiosperms, RNA-binding proteins with an RNA recognition motif (RRM)-type RNA interaction domain play an important role in developmental and environmental responses. Despite their pivotal role, a comprehensive analysis of their number and diversity has only been performed in Arabidopsis so far. Results: Here we present a detailed phylogenetic analysis of RRM-containing proteins ...

Garcı́a, Néstor H., Theresa J. Berndt, Gertrude M. Tyce, and Franklyn G. Knox. Chronic oral L-DOPA increases dopamine and decreases serotonin excretions. Am. J. Physiol. 277 (Regulatory Integrative Comp. Physiol. 46): R1476–R1480, 1999.—Given the common pathways for uptake and synthesis for dopamine and serotonin, enhanced renal dopamine synthesis in response to increased substrate 3,4-dihydroxy...

Trimethylation of lysine 4 of histone H3 occurs at the 5' end of active genes and is catalyzed by Set1 in Saccharomyces cerevisiae. Trimethylation requires histone H2B ubiquitylation and the PAF1 complex, which are linked to transcription elongation, but how they activate Set1 is not known. Set1 also bears several conserved domains with uncharacterized contributions to activity. Here, we isolat...

The spliceosomal protein SF3b49, a component of the splicing factor 3b (SF3b) protein complex in the U2 small nuclear ribonucleoprotein, contains two RNA recognition motif (RRM) domains. In yeast, the first RRM domain (RRM1) of Hsh49 protein (yeast orthologue of human SF3b49) reportedly interacts with another component, Cus1 protein (orthologue of human SF3b145). Here, we solved the solution st...

HuC is one of the RNA binding proteins which are suggested to play important roles in neuronal differentiation and maintenance. We cloned and sequenced cDNAs encoding a mouse protein which is homologous to human HuC (hHuC). The longest cDNA encodes a 367 amino acid protein with three RNA recognition motifs (RRMs) and displays 96% identity to hHuC. Northern blot analysis showed that two differen...

STUDY OBJECTIVE to assess the accuracy of nasal capnography for the monitoring of ventilation in extubated morbidly obese patients, following bariatric surgery. DESIGN prospective descriptive study. SETTING Post-anesthesia care unit. PATIENTS 25 consecutive morbidly obese patients admitted to the PACU after open bariatric surgery. INTERVENTION Patients had a nasal cannula designed to ad...

Human lymphatic filariasis (HLF) is a neglected tropical disease which threatens nearly 1.4 billion people in 73 countries worldwide. Wuchereria bancrofti is the major causative agent of HLF and it closely resembles cattle filarial parasite Setaria digitata. Due to difficulties in procuring W. bancrofti parasite material, S. digitata cDNA library has been constructed to identify novel drug targ...