We investigated the effect of functional point mutations (Q730E) endoplasmic reticulum aminopeptidase 1 (ERAP1) on proliferation, apoptosis and cytokine secretion HaCaT cells. The ERAP1 ERAP1-Q730E were overexpressed in expression TNFR1 TNFR2 cells was detected by immunofluorescence. proliferation CCK-8. Cell cycle analyzed flow cytometry. IL-1β, TNF-α IL-6 ELISA. IKKs caspase-8 Western blot. o...

White adipose tissue (WAT) is the source of proand antiinflammatory cytokines and recently, it recognized as an important source of interleukin 10 production (IL-10). Acute physical exercise induce an anti-inflammatory cytokine profile. However, the effect of chronic physical exercise in the production of IL-10 by the WAT has never been examined. IL-10 and TNF-α were assessed in WAT of rats eng...

Corresponding Author Email: [email protected] THE aim of th is s tudy was to dete rm ine phenotypic dif ferences w hen BCG in vades m acrophage s . Bacilli prepared from the sam e BCG prim ary seed, but produced in dif ferent culture m edia , w ere analysed w ith respect to th e ability to s tim ulate m acrophages and the susceptibility to tr eatm ent w ith cytokine s and n itr ic ox ide (N...

This study aims to evaluate the effect of combined use of the racemic flavanone Naringenin (NRG) and the protein sericin as TNF-α blockers. Sericin (SMs) and (R/S) NRG-loaded Sericin (SNRGMs) microparticles were prepared by spray-drying, characterized in terms of morphology and particle size distribution, and encapsulation efficiency was determined. Concerning morphology and particle size distr...

Setaria equina (S. equina) is a filarial worm that exists in peritoneal cavity of equines. This study aimed to evaluate cytokine mediators tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4) and immunoglobulin G (IgG) responses in spontaneously S. equina infected and non-infected donkeys with emphasis on choosing the best antigen that could be used in diagnosis of such filarial infection...

Selective expression of dominant negative (DN) peroxisome proliferator-activated receptor γ (PPARγ) in vascular smooth muscle cells (SMC) results in hypertension, atherosclerosis, and increased nuclear factor-κB (NF-κB) target gene expression. Mesenteric SMC were cultured from mice designed to conditionally express wild-type (WT) or DN-PPARγ in response to Cre recombinase to determine how SMC P...

TRPM2 is a Ca(2+)-permeable channel activated by oxidative stress or TNF-alpha, and TRPM2 activation confers susceptibility to cell death. The mechanisms were examined here in human monocytic U937-ecoR cells. This cell line expresses full-length TRPM2 (TRPM2-L) and several isoforms including a short splice variant lacking the Ca(2+)-permeable pore region (TRPM2-S), which functions as a dominant...

Exogenously added IL-10 rapidly inhibited Staphylococcus aureus- or LPS-induced cytokine mRNA expression in human PBMCs and monocytes, with a maximal effect observed when IL-10 was added from 20 h before until 1 h after the addition of the inducers. Nuclear run-on assays revealed that the inhibition of IL-12 p40, IL-12 p35, and TNF-alpha was at the gene transcriptional level and that the additi...