Transcriptional activators interact with multisubunit coactivators that modify chromatin structure or recruit the general transcriptional machinery to their target genes. Budding yeast cells respond to amino acid starvation by inducing an activator of amino acid biosynthetic genes, Gcn4p. We conducted a comprehensive analysis of viable mutants affecting known coactivator subunits from the Sacch...

A mutation screen in Aspergillus nidulans uncovered mutations in the acdX gene that led to altered repression by acetate, but not by glucose. AcdX of A. nidulans is highly conserved with Spt8p of Saccharomyces cerevisiae, and since Spt8p is a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex, the SAGA complex may have a role in acetate repression in A. nidulans. We used a bioinform...

TAF9 is a TATA-binding protein associated factor (TAF) conserved from yeast to humans and shared by two transcription coactivator complexes, TFIID and SAGA. The essentiality of the TAFs has made it difficult to ascertain their roles in TFIID and SAGA function. Here we performed a genomic synthetic genetic array analysis using a temperature-sensitive allele of TAF9 as a query. Results from this ...

Gene transcription, RNA biogenesis, and mRNA transport constitute a complicated process essential for all eukaryotic cells. The transcription/export factor Sus1 plays a key role in coupling transcription activation with mRNA export, and it resides in both the SAGA and TREX2 complexes. Moreover, Sus1 is responsible for GAL1 gene gating at the nuclear periphery, which is important for its transcr...

BACKGROUND There were over 5,000,000 ambulance dispatches during 2010 in Japan, and the time for transportation has been increasing, it took over 37 minutes from dispatch to the hospitals. A way to reduce transportation time by ambulance is to shorten the time of searching for an appropriate facility/hospital during the prehospital phase. Although the information system of medical institutions ...

A urbanização acelerada, desordenada e sem planejamento de Campo Grande a partir da década 1960 resultou em alterações nos processos hidrológicos geomórficos, impactando os ambientes terrestres (erosão) aquáticos (sedimentação). Este trabalho teve como objetivo mapear locais susceptíveis à erosão do solo na bacia hidrográfica córrego Bandeira, visando gerar informações que subsidiem gestores to...

An NQR Study and X-ray Structure Analysis of [H3N(CH2)3NH3]Cdl4 • 2H20, [H3CNH2(CH2)3NH3]CdBr4, [(CH3)4N]2CdBr4, and [(CH3)3S]2CdBr4* Hideta Ishihara, Keizo Horiuchi, Shi-qi Dou, Thorsten M. Gesing, J.-Christian Buhl, Helmut Paulus, and Hartmut Fuess a Faculty of Culture and Education, Saga University, Honjo-machi 1, Saga 840-8502, Japan b College of Science, University of the Ryukyus, 1 Senbar...