BACKGROUND AND OBJECTIVE Neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. The factor H binding protein (fHBP) is a key virulence factor of Neisseria meningitidis that is able to selectively bind to human factor H, the key regulator of the alternative complement pathway, which it has important implications for meningococcal pathogenesis and vaccine design. The aims o...

background: dicrocoelium dendriticum, a liver trematode of herbivores, and man is very prevalent among ruminants of iran, resulting in causing significant economic losses. recently its phenotype polymorphism has been found in iran, a phenomenon that may affect the protein profile of the parasite. objectives: the aim of this study was to determine the somatic protein profiles of d.dendriticum in...

Soybean is well recognized for having several bioactive compounds. Black soybeans have been used in Eastern countries as a healthy food and an medicinal alternative. The identification of natural substances, such peptides, present black yellow necessary. An evaluation the contribution these molecules to positive effects human health molecular studies should be done. search aspects that may expl...

In the last decade positioning of assets in a manufacturing industry has become one of the milestones in the process to increase the insight visibility and improve the manufacturing practices. The mobility of the assets in a factory makes necessary the use wireless sensor networks, but in order to avoid an increase of the complexity as well as increase of the cost the assets have to interact wi...

長年，アレルゲンの解析手法としてSDS-PAGEとWestern blottingが用いられてきた．この場合，タンパク質をできるだけ可溶化するために，SDSなどの変性剤を高濃度で添加することが多い．筆者らはこの手法を用いて，甲殻類アレルゲンの種特異的反応性の違いを検出した．ところが，別の患者血清では，この方法ではバンドが全く検出されなかった．そこでこの患者血清をdot blottingで調べたところ，生では強く反応したが，80°Cで15分加熱すると反応が失われた．そこで，生の試料をdot blotしたPVDF膜を0.1％SDSを含む電気泳動バッファー中で1時間インキュベートしたところ反応性は弱まったが，完全には反応が消失していなかった．そこで，0.1％SDSを含むSDS抽出液を用いてSDS-PAGEを行ったところ，トロポミオシンのバンドが弱く反応した．甲殻類アレルゲンのSDS変性を低...

A spoken dialog system (SDS) is a computer system which supports human-computer conversations in specific knowledge domains. It integrates technologies including speech recognition, natural language understanding, dialog modeling, language generation, and textto-speech synthesis. Advances in speech and language technologies have made SDS an important research area and have brought about systems...

Studies of the interaction of bacteria with mucus-secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far Western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed using biotinylated mucin. In addi...