Lysosome Associated Protein Transmembrane 4B (LAPTM4B) is a four-membrane spanning ceramide interacting protein that regulates mTORC1 signaling. Here, we show LAPTM4B sorted into intraluminal vesicles (ILVs) of multivesicular endosomes (MVEs) and released in small extracellular (sEVs) conditioned cell culture medium human urine. Efficient sorting ILV membranes depends on its third transmembrane...

Urinary tract infections (UTI) are among the most prevalent infectious diseases and common cause of nosocomial infections, worldwide. Uropathogenic E. coli (UPEC) responsible for approximately 80% all UTI, which commonly affect bladder. UPEC colonize urinary by ascension urethra, followed cell invasion, proliferation inside outside urothelial cells, thereby causing symptomatic quiescent intrace...

Abstract Fungal unspecific peroxygenases (UPOs) represent an enzyme class catalysing versatile oxyfunctionalisation reactions on a broad substrate scope. They are occurring as secreted, glycosylated proteins bearing haem-thiolate active site and rely hydrogen peroxide the oxygen source. However, their heterologous production in fast-growing organism suitable for high throughput screening has on...

Infectious Pancreatic Necrosis Virus (IPNV) is a member of the family Birnaviridae that has been linked to high mortalities in salmonids. Bacterial based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. VP2 is a structural viral protein of IPNV with immunogenicity effects. In this study IPNV was isolated from diseased fry ...

The type III secretion system (T3SS) encoded at the Salmonella pathogenicity island 1 (SPI-1) locus secretes protein directly from the cytosol to the culture media in a concerted, one-step process, bypassing the periplasm. While this approach is attractive for heterologous protein production, product titers are too low for many applications. In addition, the expression of the SPI-1 gene cluster...