INTRODUCTION HIV-1 is transmitted through semen from men to their sexual partners. Genital infections can increase HIV-1 RNA shedding in semen, but shedding also occurs in the absence of typical pathogens. We hypothesized that higher bacterial concentrations in semen would be associated with higher HIV-1 RNA levels. METHODS We analyzed semen samples from 42 HIV-1-seropositive Kenyan men using...

The current study was performed to test the of quality as well as the DNA integrity of preserved liquid and frozen buffalo spermatozoa due to addition of different concentrations of glutathione (0.50, 1.00, 2.00 and 3.00 mM). Liquid semen was preserved for 120 hour with or without addition of glutathione and examined for progressive motility, viability, plasma and acrosomal membranes integrity ...

Five Chios and five Friesian rams were used to study the effect of season on semen production. Semen samples were collected with an artificial vagina. In spite of the large variation of most of the semen characteristics among rams of the two breeds, seasonal variation in semen quantity (volume, concentration and total number of spermatozoa per ejaculate) and quality (percentage of motile sperma...

BACKGROUND Semen is the main vector for human immunodeficiency virus (HIV) transmission from men to women. We investigated the influence of cytokines in semen on local HIV burden and activated T cells. METHODS Blood and semen were collected from 42 HIV-negative and 38 HIV-positive men. Concentrations of 20 cytokines were measured by Luminex, and frequencies of activated T cells were measured ...

The study describes the standardization of a suitable semen cryopreservation protocol for the first time in mithun (Bos frontalis) and birth of the first mithun calf through artificial insemination. The semen samples were collected from adult bulls through the rectal massage method and cryopreserved in liquid nitrogen using tris-egg yolk-glycerol diluent. The diluted semen samples were packaged...

Introduction Primary breeders are currently faced with the same problem plaguing most animal industries, rising production costs. Labor, feed and energy costs are forcing primary breeders to consider more economical ways of maintaining gene pools. One way to reduce such costs is with the use of frozen semen. With frozen semen available from superior sires, primary breeders will have the tool to...

Although abnormal semen parameters are generally considered to be poor predictors of fertility, Semen Analysis (SA) parameters are often used as a screening test to detect male factor infertility. Certainly, men with azoospermia or severe oligozoospermia have markedly impaired fertility potential [1-3]. Since men with abnormal fertility could undergo various medical or surgical treatments to im...

CONTEXT AND OBJECTIVE Recent studies have shown regional and population differences in semen characteristics. The objective was to establish reference values for semen analysis and to verify the effect that age, length of sexual abstinence, seasonality, smoking habits and coffee consumption have on fertile individuals' semen characteristics. DESIGN AND SETTING Prospective study in the Urology...

MicroRNAs (miRNAs) play a crucial role in tissue development and the pathology of many diseases, however, the effects and roles of miRNAs in the development of semen abnormalities in infertile males have not yet been investigated. In this study, we analyzed and compared the miRNA expression profiles of abnormal semen from 86 infertile males with normal semen from 86 healthy males using an miRNA...

this study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (ivf) of goat oocytes. cumulus oocytes complexes (cocs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. upon grading, only normal quality cocs were maturated in tcm-199 for 48 hours. the percentage of cocs reached to the m-ii sta...