MEMS micro-T-switches actuated via electrochemical bubbles for cell sorting applications in a monolithic chip level are proposed and successfully demonstrated. The electrolysis-bubble actuator, which has the features of low operation temperature and high surface-tension force, is developed to actuate the micro-T-switch sorting structure in our device. The double T-structure design, the T-shape ...

OBJECTIVE To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate. DESIGN Prospective-controlled study. SETTING Andrology department at a university-based medical institution. PATIENT(S) Ten healthy volunteer sperm donors. INTERVENTION(S) Sperm populations were separated using annexin-V magnetic-activated ce...

Eukaryotes are characterized by endomembranes that are connected by vesicular transport along secretory and endocytic pathways. The compositional differences between the various cellular membranes are maintained by sorting events, and it has long been believed that sorting is based solely on protein-protein interactions. However, the central sorting station along the secretory pathway is the Go...

The spectral distribution of music audio has an important influence on listener perception, but large-scale characterizations are lacking. Therefore, the long-term average spectrum (LTAS) was analyzed for a large dataset of popular music. The mean LTAS was computed, visualized, and then approximated with two quadratic fittings. The fittings were subsequently used to derive the spectrum slope. B...

The majority of tumors, including melanoma, are phenotypically heterogeneous in that they contain various cell populations with differential expression of cell surface antigens such as CD133/Prominin-1. We have used fluorescence-activated cell sorting (FACS) technology to purify CD133(+) and CD133(-) cellular subsets from mouse melanoma models for high-quality total RNA practical for downstream...

The developing central nervous system of vertebrates contains an abundant cell type designated radial glial cells. These cells are known as guiding cables for migrating neurons, while their role as precursor cells is less clear. Since radial glial cells express a variety of astroglial characteristics and differentiate as astrocytes after completing their guidance function, they have been consid...

Rapid and e$cient sorting is important for progenitor cell isolation. Therefore, we have built and evaluated a high-speed, continuous #ow, quadrupole magnetic sorter. The cross-over behavior of non-magnetic particles, combined with separation theory is used to optimize performance. CD34# progenitor cells are separated to purities from 64}95% and a throughput of 10 cells/s. 2001 Elsevier Science...

Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell...

Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe J...

spike sorting is a class of techniques used in the analysis of electrophysiological data. studying the dynamics of neural activity via electrical recording relies on the ability to detect and sort neural spikes recorded from a number of neurons by the same electrode. this article reviews methods for detecting and classifying action potentials, a problem commonly referred to as spike sorting.