The 0.5 M KC1 wash fraction of rabbit liver microsomes has been fractionated by DEAE-cellulose chromatography into the protein synthesis initiation factors IF-Ml, IF-M~(~+BJ, and IF-MB. IF-M2cA+~) was also separated into two components, IF-MZA and IF-MOB, by Sephadex G-200 chromatography. Further purification of the factors was obtained by ammonium sulfate fractionation, ion exchange chromatogr...

Theoretical expressions are derived for affinity chromatography of systems comprising an acceptor A with one binding site for attachment to a functional group X on the column matrix and one site for interaction with a small ligand B that specifically affects its elution. From a general relationship covering all possible interactions between A, B and X simpler expressions are derived for affinit...

Ca2+-activated protein phosphatase activity was demonstrated in mouse pancreatic acinar cytosol with alpha-casein and skeletal-muscle phosphorylase kinase as substrates. This phosphatase activity preferentially dephosphorylated the alpha subunit of phosphorylase kinase. After DEAE-cellulose chromatography, the Ca2+-activated phosphatase activity became dependent on exogenous calmodulin for maxi...

A naturally occurring inhibitor of activated blood coagulation Factor X has been isolated from pooled rabbit plasma and purified by the combination of Sephadex G-ZOO gel filtration, DEAE-Sephadex A-SO, and DEAE-cellulose chromatography. On microzone electrophoresis the purified product traveled as an az-globulin, and on 7.5% polyacrylamide gel disc electrophoresis at pH 9.5 it traveled as a sin...

1. DNA polymerase from nuclear and supernatant fractions of cultured mouse L929 cells was fractionated on columns of Sephadex G-200, Sepharose 4B and of DEAEcellulose. Several peaks of activity are found on Sephadex chromatography and the distribution of activity between these depends on: (a) the source of the enzyme, i.e. nuclear or supernatant fraction; (b) the mode of extraction of the enzym...

CM-Sephadex chromatography (phosphate buffer, l o r n , pH7.4) resolved the lithocholicacid-binding mixture obtained fromDEAE-Sephadex into two fractions, both able to bind lithocholic acid and both having glutathione S-transferase activity. Agarose and polyacrylamide-gel electrophoresis of the fractions obtained from CM-Sephadex chromatography demonstrated the presence of only a single protein...

1. Cell-free culture filtrates from Trichoderma koningii were concentrated by precipitation with ammonium sulphate between the limits of 20% and 80% saturation. 2. Removal of a low-molecular-weight carboxymethylcellulase (CM-cellulase) component by chromatography on Sephadex G-75 had no effect on the ability of the enzyme complex to solubilize cotton. 3. Further chromatography on DEAE-Sephadex ...

Soluble, partially purified, histocompatibility antigens that were obtained from the membranes of A/J spleen cells have been assayed for their capacity to elicit immunologic enhancement of two tumors of A-strain origin: YAA-C1 and Sarcoma I. Crude membrane material and a partially purified, soluble antigen that were contained in a specific fraction, obtained after chromatography on a Sephadex G...

GSH-dependent enzymic reduction of dehydroascorbic acid to ascorbic acid has been studied in rat liver cytosol. After gel filtration of cytosol on Sephadex G-100 SF, dehydroascorbate reductase activity was recovered in two distinct peaks, one corresponding to glutaredoxin (an enzyme already known for its dehydroascorbate reductase activity) and another, much larger one, corresponding to a novel...

We have isolated and purified to homogeneity an alpha,alpha'-trehalose 6-monomycolate:alpha,alpha'-trehalose mycolyltransferase (trehalose mycolyltransferase) from Mycobacterium smegmatis that catalyzes the exchange of a mycolyl group between trehalose, trehalose 6-monomycolate (TM), and trehalose 6,6'-dimycolate (TD). This enzyme was prominent in M. smegmatis and it catalyzed the following rea...