The contamination of ores with wastes or materials of lower than the cut-off grade is referred to as dilution. Dilution is an undesirable phenomenon that, on one hand, reduces the product grade and, consequently, reduces the sales prices and, on the other hand, adds an extra cost to waste production. Therefore, studying and evaluating the dilution risk is important in mining, and especially in ...

The decay of signal over time was performed using serial dilution of a E. coli cell lysate containing a 51 kD polyhistidinetagged recombinant protein run on a 4–20% linear gradient Criterion gel. Blots were probed with mouse anti-polyhistidinetag primary antibody followed by incubation with GAM-HRP secondary antibody and StrepTactin HRP (1:10,000) for 1 hr each. The blots were then cut into two...

Actinomycetes were isolated from eight samples of two different habitats, i.e., garden soil and cultivated field soil. Isolation of actinomycetes was carried out on soil extract agar medium using serial dilution method and forty seven isolates were obtained. All these actinomycetes were then assessed for their antifungal activity against two fungal pathogens i.e., Candida albicans and Aspergill...

The present study was carried out to know the lowest detection limit of Listeria monocytogenes by PCR. The quantification of organisms was done by CFU counting from ten fold serial dilution and PCR amplification of inlA gene fragment was performed from each dilution. The PCR could detect as low as 20 organisms indicating the lowest PCR detection limit. Thus, lowest number of L. monocytogenes de...

the aim of this work was to study the antimicrobial activity of tomato skin lycopene oleoresin against pseudomonas aeruginosa، escherichia coli، staphylococcus ureuse، salmonella typh ، l. monocytogenes ، bacillus cereus، bacillus licheniformis. oleoresin was extracted from tomato peel. lycopene content was measured by spectrophotometer. lycopene oleoresin contained 2321mg lycopene/ 100 g oleor...

This paper gives an overview of several (mostly recent) statistical contributions to the theory of Limiting and Serial Dilution Assays (LDA's, SDA's). A simple and useful method is presented for the setup of a design for an LDA or an SDA. This method is based on several user-supplied design parameters, consisting in the researcher's advance information and other parameters inherent to the parti...

staphylococcus spp., is a common bacteria in cattle mastitis. detection of the low number of bacteria requires a sensitive method. we used multiplex polymerase chain reaction (m-pcr) assay to detect the genes encoding sea, seb, sec toxins, that are specific in s. aureus bacteria. the gene of 23s rrna that is conserved in all staphylococcus spp., also was used. due to the stiffness and low effec...