The surfactant fraction (55,000-g pellet) of leukocyte-free rat bronchoalveolar lavage fluid contains factors that rapidly kill and lyse pneumococci. These factors were purified and identified biochemically by using a quantitative bactericidal test to monitor fractionation procedures. 91% of the antipneumococcal activity of rat surfactant was recovered in chloroform after extraction of rat surf...

Although silicic acid jellies are inferior to agar, gelatin, blood serum or egg white in the preparation of solid media for the majority of bacteriological requirements, there are certain specialized purposes, such as the isolation of autotrophic bacteria, for which an inorganic jelly is necessary. Theoretical advantages from the utilization of silica gel as a specialized substitute for organic...

The surfactant fraction (55,000-g pellet) of leukocyte-free rat bronchoalveolar lavage fluid contains factors that rapidly kill and lyse pneumococci. These factors were purified and identified biochemically by using a quantitative bactericidal test to monitor fractionation procedures. 91% of the antipneumococcal activity of rat surfactant was recovered in chloroform after extraction of rat surf...