Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to sperm after repeated freezing and thawing. The results showed that normozoospermic and oligozoospermic sperm survive...

During winter, larvae of the Antarctic midge, Belgica antarctica (Diptera, Chironomidae), must endure 7-8 months of continuous subzero temperatures, encasement in a matrix of soil and ice, and severely desiccating conditions. This environment, along with the fact that larvae possess a high rate of water loss and are extremely tolerant of desiccation, may promote the use of cryoprotective dehydr...

Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the conformation of biomacromolecule and/or spin-label. We studied in detail the effect of these experimental variables on the distance distributions obtained by DEER from a se...

Abstract Study question Is large for gestational age (LGA) observed in singletons born following frozen embryo transfers (FET) either due to freezing technique or endometrial preparation protocol? Summary answer Artificial cycles were associated with a higher rate of LGA when compared ovulatory cycles, whereas no difference was between the techniques. What is known already Several studies neona...

A widely discussed topic in biological applications is the impact of the sample freezing process and the final holding and storage temperatures on the stability of biological samples. In order to preserve biological material in its optimal condition, a slow freezing process with storage temperatures held to the absolute practical minimum is essential. While these guidelines are generally recogn...

Heparinized whole blood, parasitized with Aegyptianella pullorum, was collected from 2 fowls. Buffered lactose peptone (BLP) was added v/v as a stabilizer and the mixture lyophilized in 2 ml aliquots after rapid or slow freezing. At different stages during the freeze-drying process, as well as after lyophilization and reconstitution with 1.8 ml of sterile water, samples were taken and injected ...

Plasma was frozen and stored in different ways before processing to cryoprecipitate by a standard thawing technique. Freezing rate was found to be important with slow freezing having a deleterious effect on cryoprecipitate quality. Storage of frozen plasma at constant temperatures for periods up to six months had no effect on the quality of cryoprecipitate, with no difference being found for pl...

Oocyte cryopreservation for fertility preservation, in both medical and elective situations, has significantly increased as freezing technology has improved. Slow freezing techniques demonstrated ∼ 50-80% survival of mature oocytes, however vitrification with ∼ 97% survival has become the preferred method for oocyte cryopreservation around the world. Our work investigated the effect of transpor...

We consider a heat conduction model introduced in [2]. This is an open system in which particles exchange momentum with a row of (fixed) scatterers. We assume simplified bath conditions throughout, and give a qualitative description of the dynamics extrapolating from the case of a single particle for which we have a fairly clear understanding. The main phenomenon discussed is freezing, or the s...