La investigación hace un recorrido desde la creación de Bellavista en década los noventa, como una iniciativa que combina conservación y el ecoturismo, hasta su declaratoria primera área protegida privada del Sistema Nacional Áreas Protegidas (SNAP) 2019, siguiendo procedimiento técnico-normativo establecido por Autoridad Ambiental. El objetivo fue documentar proceso llevó a propietarios conser...

A snap-stabilizing protocol guarantees that the system always behaves according to its specification provided some processor initiated the protocol. We present how to snap-stabilize some important protocols, like Leader Election, Reset, Snapshot, and Termination Detection. We use a Snap-stabilizing Propagation of Information with Feedback protocol for arbitrary networks as the key module in the...

We have examined the effect of alpha-SNAP on exocytosis in adrenal chromaffin cells by direct assay of exocytosis using patch-clamp capacitance analysis. Cells were recorded using the whole cell patch-clamp configuration and the cells dialysed with control pipette solution or with a pipette solution containing alpha-SNAP or the deletion mutant alpha-SNAP(41-295). The deletion mutant was found t...

The core human U6 promoter consists of a proximal sequence element (PSE) located upstream of a TATA box. The PSE is recognized by the snRNA-activating protein complex (SNAP(c)), which consists of five types of subunits, SNAP190, SNAP50, SNAP45, SNAP43, and SNAP19. The TATA box is recognized by TATA box binding protein (TBP). In addition, basal U6 transcription requires the SANT domain protein B...

Movement through the endocytic pathway occurs principally via a series of membrane fusion and fission reactions that allow sorting of molecules to be recycled from those to be degraded. Endosome fusion is dependent on SNARE proteins, although the nature of the proteins involved and their regulation has not been fully elucidated. We found that the endosome-associated hepatocyte responsive serum ...

The assembly of four soluble N-ethylmaleimide-sensitive factor attachment protein receptor domains into a complex is essential for membrane fusion. In most cases, the four SNARE-domains are encoded by separate membrane-targeted proteins. However, in the exocytotic pathway, two SNARE-domains are present in one protein, connected by a flexible linker. The significance of this arrangement is unkno...

The assembly of multimeric protein complexes that include vesicle-associated membrane protein 2 (VAMP-2) and the plasma membrane proteins syntaxin 1A and synaptosome-associated protein of 25 kDa (SNAP-25) are thought to reflect the biochemical correlates of synaptic vesicle targeting, priming, or fusion. Using a variety of protein-protein interaction assays and a series of deletion and point mu...