نتایج جستجو برای: snps typing
تعداد نتایج: 45092 فیلتر نتایج به سال:
Allele-specific polymerase chain reaction (AS-PCR) is a rapid and cost-effective single nucleotide polymorphism (SNP) genotyping method compared to multiplex or real-time PCR. The SNPs occurring in mitochondrial DNA (mtSNPs) the most abundant humans can be used human identification involving mass fatality incidents. Nevertheless, application of AS-PCR has yet widely applicable forensic investig...
Introduction. Bacillus anthracis is the causative agent of anthrax, a pathogen characterized by high genetic monomorphism that complicates differentiation strains. Thus, molecular methods for typing require improvement.
 The aim study. To select marker SNPs new groups B. and to develop method their laboratory identification using HRM PCR.
 Materials methods. core genome 222 strains fr...
Objectives. Epidemiological data concerning third-generation cephalosporin (3GC) resistance in wild fauna are scarce. The aim of this study was to characterize the genes, their genetic context, and clonal relatedness 17 Escherichia coli resistant 3GC isolated from animals. Methods. isolates were characterized by short-read whole genome sequencing, long-read sequencing used for hybrid assembly p...
Background:It has been hypothesized that genetic factors other than histocompatibility disparity may play a role in predisposition to developing Chronic Myelogenous Leukemia (CML). In this regard, Th1 and Th2 cytokines and their gene polymorphism seems to be important. Overall expression and secretion of cytokines is dependent, at least in part, on genetic polymorphism (nucleotide variations) w...
There is increasing evidence that strain variation in Mycobacterium tuberculosis complex (MTBC) might influence the outcome of tuberculosis infection and disease. To assess genotype-phenotype associations, phylogenetically robust molecular markers and appropriate genotyping tools are required. Most current genotyping methods for MTBC are based on mobile or repetitive DNA elements. Because these...
BACKGROUND Plague is a life-threatening disease caused by the bacterium, Yersinia pestis. Since the 1990s, Africa has accounted for the majority of reported human cases. In Uganda, plague cases occur in the West Nile region, near the border with Democratic Republic of Congo. Despite the ongoing risk of contracting plague in this region, little is known about Y. pestis genotypes causing human di...
Immigrants from regions with a high incidence of tuberculosis (TB) are a risk group for TB in low-incidence countries such as Switzerland. In a previous analysis of a nationwide collection of 520 Mycobacterium tuberculosis isolates from 2000 to 2008, we identified 35 clusters comprising 90 patients based on standard genotyping (24-locus mycobacterial interspersed repetitive-unit-variable-number...
an important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. nowadays, the use of compounds such as essential oils (eos) and nanoparticles is applicable in microbiology studies. the main objective of this experiment was to study the s...
Forensic science is basically the determination of the human identity. With advances in science and technology and the availability of DNA profiling, it is possible now to determine the identity even in decomposed and mutilated bodies and even with parts of a body as trivial as a hair or a drop of a blood. Methods and materials: Short tandem repeat polymorphism (STRP) genotyping and single nucl...
Introduction: Silver nanoparticles (SNPs) have recently been suggested to increase the antimicrobial properties of soft liner materials. However, their safety remains a matter of debate. This study aimed to evaluate the cytotoxicity of Mucopren® soft silicone liner material (Mucopren; Kettenbach, Germany) incorporated in SNPs. Methods: The SNPs with ...
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