cryopreservation is as an effective way to prevent species extinction risk. in this study, we have investigated the protective effect of different concentrations cryoprotectant and effect of different dilution ratios on sperm motility percent and its motility duration after thaw. for this work dimethyl sulfoxide, methanol and ethylene glycol was used as cryoprotectant, each at concentration of ...

When sperm of Strongylocentrotus purpuratus or Lytechinus pictus are diluted into seawater, motility is initiated; and when exposed to egg jelly, an acrosome reaction is induced. In the presence of a variety of structurally different metal chelators (0.1-1 mM EDTA, EGTA, phenanthroline, dipyridyl, cysteine, or dithiothreitol), motility initiation is delayed and the acrosome reaction is inhibite...

In the present study, we evaluated sustaining effect of various glutathione (GSH) concentrations in extender on rabbit sperm attributes during storage at 5°C for 24 h. Semen was collected from regular donor bucks using an artificial vagina and initially quality. The qualifying ejaculates were diluted with one extenders having 0, 1, 2, 4 or 8 mM GSH, to achieve a final concentration 1×108 sperm/...

The Effect of histamine and some Antihistamine in the motitlity of human semin were studid. It shows that the histamine increases sperm metility, further more H2 antihistamine crimetidine had no effect on sperm motility and classical antihistamines decreased sperm motility , where as the inhibition produced by H antihistamine could be over comed with increasing concentration of histamine. Final...

Several gene expressions are related to the success of spermatogenesis. Protamine plays an important role in sperm DNA packaging, spermatogenesis, and quality. This study aimed isolate RNA from spermatozoa determine expression profiles protamine 1 (PRM1) 2 (PRM2). Six Pasundan bulls aged 5-8 years with a body weight 380 kg-430 kg were used for this study. The classified into group A (70%-79%) B...

The Sperm-Class Analyzer detected four subpopulations of spermatozoa with different motility characteristics in the ejaculate of the Catalonian donkey. Significant differences (P < 0.001) in the distribution of these subpopulations, as well as in total sperm number and percentage total motility, were seen in the diluted semen of four sampled donkeys. All the ejaculates evaluated showed excellen...

Introduction: tegrity is the most important component for transferring genetic information Sperm DNA fragmentation is considered a component of male infertility. Its assessment comprises conventional semen analysis of which emphasis is placed on its evaluation in fertility clinics. In this study, we investigate the relationship between sperm parameters of motility, morphology, concentratio...

Background: Facing infertility put too much emotional stress and physical burden on infertile couples that make it difficult to deal with. According to this fact that infertility in some men appears with specified disorder but in most cases there is no clear cause for male infertility which determine the special attention to lifestyle and environmental factors. In this study the effects of smok...

Epidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not been published. We analyzed motility parameters of ejaculates after 24, 48, and 72 hours from the EG...