We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons. We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers. MVs isolated from all 3 culture conditions were incubated with 661W ce...

After the discovery of functionally superior Tie2-positive nucleus pulposus (NP) progenitor cells, new methods were needed to enable mass culture and cryopreservation maintain these cells in an undifferentiated state with high cell yield. We used six types EZSPHERE® dishes, which support spheroid-forming colony culture, examined NP spheroid-formation ability, number, proliferation, mRNA express...

This work highlights the importance of surface modification on quality and quantity spheroid formation microfluidic-based biochips.

Abstract Spheroids have become essential building blocks for biofabrication of functional tissues. Spheroid formats allow high cell-densities to be efficiently engineered into tissue structures closely resembling the native In this work, we explore assembly capacity cartilaginous spheroids ( d ? 150 µ m) in context endochondral bone formation. The fusion at various degrees differentiation was i...

In this paper, we demonstrate formation of size-controlled 3-dimensional hetero-spheroids using a micro-fluidic device in approximately 2 min. The device collects cells in the center of a micro-chamber using micro-rotational flow as shown in Fig. 1. In previous work, we used this device to generate size-controlled HepG2 spheroids [1]. We applied it to form heterospheroids consisting of HepG2 an...

background: rad51 is a protein with critical role in double strand break repair. down-regulation of this protein has a significant effect in radiosensitivity of some cell lines like prostate carcinoma. compared to monolayer cell culture model, the spheroids are more resistant to radiation. the aim of the current study was to determine the rad51 protein level in du145 spheroids, and monolayer ce...

We review recent determinations of the present day mass function (PDMF) and initial mass functions (IMF) in various components of the Galaxy, disk, spheroid, young and globular clusters and in conditions characteristic of early star formation. As a general feature, the IMF is found to depend weakly on the environment and to be well described by a power-law form for m & 1M⊙ and a lognormal form ...

We propose a neural probe combined with a neuronal spheroid (neurospheroid) cultured on electrodes for deep brain stimulation. Since neurons of the spheroid extend their axons deep into the brain tissue, the neural probe can electrically stimulate deep brain area via the neurons without inserting an invasive probe. Here we first fabricated an electrode and then successfully placed a neurospheor...

This paper reports a microfluidic device composed of a reversibly-assembled microfluidic chamber chip and multi-microwell array chip. This device enabled perfusion culture of spheroid in a microarray format, and detailed post-culture analysis by collection of spheroids from the disassembled device. The device provides four advantages simultaneously; the uniform spheroid formation, growth analys...